In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Target: NAD+-dependent type-I 15-hydroxy PGDH
IC50: 26.4 nM
Ki: 5 nM
STK393606 is a competitive inhibitor of NAD+-dependent type-I 15-hydroxy PGDH, with the IC50 value of 26.4±2.4 nM, and the Ki value of 5 nM, which could cause a maximum of 100% inhibition .
Hydroxyprostaglandin dehydrogenase 15-(NAD), also called 15-hydroxy prostaglandin dehydrogenase (15-hydroxy PGDH), is a critical enzyme participated in the inactivation of prostaglandins (PGs) and related eiscosanoids. 15-hydroxy PGDH belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of substrates with NAD+ or NADP+ as acceptor. 15-hydroxy PGDH could catalyze the oxidation of primary PGs to their 15-keto metabolites . Radioimmunoassay for 15-hydroxy prostaglandin dehydrogenase had identified two types of enzyme activity, of which type-I used NAD+ as a cofactor, while type-II used NADP+ as a cofactor and bear wider substrate specificity .
In Vitro: no data available.
In Vivo: no data available.
Clinical trial: no data available.
 Niesen F H, Schultz L, Jadhav A, et al. High-Affinity Inhibitors of Human NAD + -Dependent 15-Hydroxyprostaglandin Dehydrogenase: Mechanisms of Inhibition and Structure-Activity Relationships[J]. PLOS ONE, 2010, 5(11).
 Tai H, Ensor C M, Tong M, et al. Prostaglandin catabolizing enzymes.[J]. Prostaglandins & Other Lipid Mediators, 2002: 483-493.
 Lee S C, Levine L. Prostaglandin metabolism. II. Identification of two 15-hydroxyprostaglandin dehydrogenase types.[J]. Journal of Biological Chemistry, 1975, 250(2): 548-552.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤12mg/ml in DMSO;14mg/ml in dimethyl formamide|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|