StemRegenin 1 (hydrochloride)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 127 nM in CD34+ cells
StemRegenin 1 is an aryl hydrocarbon receptor signaling antagonist.
The aryl hydrocarbon receptor (AhR ) is a protein that is encoded by the AHR gene. The AhR is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic (aryl) hydrocarbons.
In vitro: In previous study, the unbiased screen with primary human HSCs identified the StemRegenin 1 (SR1), which could promote the ex-vivo expansion of CD34+ cells. In addition, the culture of HSCs with SR1 resulted in a 50-fold increase in cells expressing CD34. Moreover, the mechanistic studies showed that SR1 acted by antagonizing the AhR .
In vivo: In aninmal study, 300 to 30,000 uncultured CB CD34+ cells were transplanted into NSG mice. Results showed that CB CD34+ cells cultured with SR1 led to significant increases in human engraftment in the peripheral blood at 1 week, compared with mice transplanted with 10,000 uncultured cells or the progeny of 10,000 control cells .
Clinical trial: An open label, randomized phase II trial comparing SR-1 cultured umbilical cord blood to unmanipulated umbilical cord blood transplantation after a myeloablative CY/FLU/TBI conditioning has been proposed, but is not yet open for participant recruitment .
 A. E. Boitano, J. Wang, R. Romeo, et al. Aryl hydrocarbon receptor antagonists promote the expansion of human hematopoietic stem cells. Science 329,1345-1348 (2010).
 https://clinicaltrials. gov/ct2/show/NCT02765997 term=StemRegenin+1&rank=1
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Formula||C24H23N5OS • HCl|
|Solubility||≤5mg/ml in DMSO;5mg/ml in dimethyl formamide|
|Chemical Name||4-(2-((2-(benzo[b]thiophen-3-yl)-9-isopropyl-9H-purin-6-yl)amino)ethyl)phenol, monohydrochloride|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|