In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
STAT5 Inhibitor is a cell-permeable inhibitor of STAT5 with IC50 value of 47 μM against STAT5β SH2 domain EPO peptide binding activity .
Signal Transducers and Activators of Transcriptions (STATs) are a family of transcription factors that are primarily activated by membrane receptor-associated Janus kinases (JAK) in response to a large number of extracellular signaling molecules including interferons, cytokines, and growth factors, and mediate many aspects of cellular immunity, proliferation, apoptosis and differentiation . STAT5 consists of two isoforms STAT5α and STAT5β and is overactive in several kinds of cancers .
STAT5 Inhibitor is a cell-permeable STAT5 inhibitor. STAT5 Inhibitor inhibited STAT5β SH2 domain EPO peptide binding activity with IC50 value of 47 μM and exhibited less potency inhibiting the SH2 domain of STAT1, STAT3, and LCK by 36%, 26% and 7% at 500 μM, respectively . In lysates from K562 cells, STAT5 Inhibitor reduced the concentration of STAT5/STAT5/DNA complex in a dose-dependent way .
. Müller J, Sperl B, Reindl W, et al. Discovery of chromone-based inhibitors of the transcription factor STAT5. Chembiochem. 2008 Mar 25;9(5):723-7.
. Leonard WJ, O'Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol. 1998;16:293-322.
. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem. 1995;64:621-51.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Synonyms||Signal Transducers and Activators of Transcription 5|
|Solubility||≤5mg/ml in DMSO;2mg/ml in dimethyl formamide|
|Chemical Name||2-[(4-oxo-4H-1-benzopyran-3-yl)methylene]hydrazide 3-pyridinecarboxylic acid|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|