Recombinant Human IL-2
Interleukin-2 (IL2), also known as a T-cell growth factor, TCGF, and Aldesleukin, is a secreted protein that belongs to the IL-2 family. IL2 has potent stimulatory activity for antigen-activated T cells, and is expressed by T cells, B cells, dendritic cells, and eosinophils [1-3]. Mature human IL-2 shares 56% aa sequence identity with mouse IL-2. Human and mouse IL-2 exhibit cross-species activity [4]. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes [5-7]. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis [1,2]. It contributes to T cell homeostasis by promoting the Fas-induced death of naive CD4+ T cells but not activated CD4+ memory lymphocytes [8]. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells [9-11]. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity [12,13].
Reference
[1]. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
[2]. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
[3]. Taniguchi, T. et al. (1983) Nature 302:305.
[4]. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
[5]. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
[6]. Wang, X. et al. (2005) Science 310:1159.
[7]. Bodnar, A. et al. (2008) Immunol. Lett. 116:117
[8]. Jaleco, S. et al. (2003) J. Immunol. 171:61.
[9]. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
[10]. Laurence, A. et al. (2007) Immunity 26:371.
[11]. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
[12]. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
[13]. Fehervari, Z. et al. (2006) Trends Immunol. 27:109
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Accession # |
P60568 |
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Alternate Names |
Human IL2; IL-2; IL-2; IL2; interleukin-2 |
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Source |
Human embryonic kidney cell, HEK293-derived human IL2 protein |
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Protein sequence |
Ala21-Thr153 |
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M.Wt |
15.4 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer to a concentration of 0.2 mg/mL |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The EC50 for this effect is 0.05-0.25 ng/mL. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.