In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Pregnenolone-16α-carbonitrile is a rodent pregnane X receptor (PXR) activator involved in inducing the synthesis of a unique cytochrome P450 peptide in hepatic microsomes of male rats .
The nuclear pregnane X receptor (PXR) is an important component of the body’s adaptive defense mechanism against toxic substances. PXR could be activated by a large number of endogenous and exogenous chemicals such as antibiotics, steroids, antimycotics, and bile acids. PXR acts as a generalized sensor of hydrophobic toxins. PXR heterodimer binds with the 9-cis retinoic acid receptor (NR2B) to DNA response elements in the regulatory regions of cytochrome P450 3A monooxygenase genes and a number of other genes involved in the metabolism and elimination of xenobiotics from the body .
In vitro: In rat HSCs, PCN inhibited the trans-differentiation of rat HSCs in vitro despite the absence of PXR expression .
In vivo: PCN administration (25 mg/kg; one injection/week) to rats significantly increased the relative liver weight. In rats, PCN administration did not result in liver damage or significantly affect the level of liver damage caused by carbon tetrachloride. PCN treatment to carbon tetrachloride-treated rats resulted in a significant decrease in both intralobular α-smooth-muscle-actin immunostaining and intense Sirius Red staining in liver sections. PCN didn’t interfere with the metabolism of carbon tetrachloride to toxic metabolites .
 Birnbaum L S, Baird M B, Massie H R. Pregnenolone-16alpha-carbonitrile-inducible cytochrome P450 in rat liver[J]. Research communications in chemical pathology and pharmacology, 1976, 15(3): 553.
 Kliewer S A, Goodwin B, Willson T M. The nuclear pregnane X receptor: a key regulator of xenobiotic metabolism[J]. Endocrine reviews, 2002, 23(5): 687-702.
 Marek C J, Tucker S J, Konstantinou D K, et al. Pregnenolone-16α-carbonitrile inhibits rodent liver fibrogenesis via PXR (pregnane X receptor)-dependent and PXR-independent mechanisms[J]. Biochemical Journal, 2005, 387(3): 601-608.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤1mg/ml in ethanol;1mg/ml in methanol;1mg/ml in acetonitrile|
|Canonical SMILES||O[[email protected]](C1)CC[[email protected]@]2(C)C1=CC[[email protected]]3([H])[[email protected]]2([H])CC[[email protected]@]4(C)[[email protected]@]3([H])C[[email protected]@H](C#N)[[email protected]@H]4C(C)=O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|