In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Picrotoxin is a noncompetitive antagonist of γ-aminobutyric acid-A (GABAA) and GABAC receptors, with IC50 value of 0.6 μM for GABAC receptors [1, 2].
Three classes of GABA receptors have been characterized based on their pharmacology, namely GABAA, GABAB and GABAC receptors, among which GABAA and GABAC receptors are ligand-gated chloride (Cl-) channels. GABA receptors play important roles in central nervous system by responding to the inhibitory neurotransmitter GABA .
In hippocampal cultured neurons, picrotoxin blocked GABAA-mediated currents, with an IC50 of 14 μM. Bath application of picrotoxin at 7 μM induced epileptiform activity, by blocking 30% of the GABAA-mediated receptor responses .
In male rats, i.p. injection (3 mg/kg) of picrotoxin significantly increased somatostatin (SRIF) release from the median eminence (ME) compared to baseline levels measured in the same animals, whilst local perfusion of picrotoxin did not affect SRIF release from the ME, which suggested a physiological endogenous inhibitory tone of GABA on SRIF release .
. Gibbs III J W, Sombati S, DeLorenzo R J, et al. Physiological and pharmacological alterations in postsynaptic GABA(A) receptor function in a hippocampal culture model of chronic spontaneous seizures. Journal of Neurophysiology, 1997, 77(4): 2139-2152.
. Goutman J D, Calvo D J. Studies on the mechanisms of action of picrotoxin, quercetin and pregnanolone at the GABA rho 1 receptor. British Journal of Pharmacology, 2004, 141(4): 717-727.
. Arancibia S, Briozzo P. Peripheral administration of picrotoxin and bicuculline stimulates in vivo somatostatin release from rat median eminence. Neuroscience Letters, 1990, 111(1-2): 211-216.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥24.55 mg/mL in DMSO; ≥2.3 mg/mL in H2O with gentle warming and ultrasonic; ≥45.2 mg/mL in EtOH|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|