HyperPFU™ high-fidelity DNA polymerase
HyperPFU™ high-fidelity DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, it produces PCR products with very high speed and accuracy. Also, HyperPFU™ high-fidelity DNA polymerase is tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. It becomes the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
HyperPFU™ high-fidelity DNA polymerase is important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult amplicons. It is one of the most accurate thermostable polymerases available, with an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase. It possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperPFU™ high-fidelity DNA polymerases is supplied with standard 5X HF Buffer, which can be used for complex or GC-rich templates.
- 1. Hui Tang, Dan Li, et al. "Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction." Mediators Inflamm. 2024 Apr 5:2024:9986187. PMID: 38716374
- 2. Qiao Jin, Chiyuan Zhang, et al. "Quinic acid regulated TMA/TMAO-related lipid metabolism and vascular endothelial function through gut microbiota to inhibit atherosclerotic." J Transl Med. 2024 Apr 15;22(1):352. PMID: 38622667
- 3. Qing Wang, Zhiyi He, et al. "Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice." BMC Biotechnol. 2024 Mar 8;24(1):13. PMID: 38459479
- 4. Yongjian Li, Zhengping Tan, et al. "Rutaecarpine ameliorates imiquimod-induced psoriasis-like dermatitis in mice associated with alterations in the gut microbiota." Acta Biochim Biophys Sin (Shanghai). 2024 Mar 25;56(3):345-355. PMID: 38419497
- 5. Yao Zou, Wei Xiao, et al. "Human umbilical cord mesenchymal stem cells improve disease characterization of Sjogren's syndrome in NOD mice through regulation of gut microbiota and Treg/Th17 cellular immunity." Immun Inflamm Dis. 2024 Jan;12(1):e1139. PMID: 38270310
- 6. Ruoxia Wu, Jiaqing Xiong, et al. "Quercetin/Anti-PD-1 Antibody Combination Therapy Regulates the Gut Microbiota, Impacts Macrophage Immunity and Reshapes the Hepatocellular Carcinoma Tumor Microenvironment." Front Biosci (Landmark Ed). 2023 Dec 1;28(12):327. PMID: 38179731
- 7. Pablo Rodríguez-Fernández, Laure Botella, et al. "MptpB Inhibitor Improves the Action of Antibiotics against Mycobacterium tuberculosis and Nontuberculous Mycobacterium avium Infections." ACS Infect Dis. 2023 Dec 12. PMID: 38085851
- 8. Wen Sheng, Wenjing Xu, et al. "Guijiajiao (Colla Carapacis et Plastri, CCP) prevents male infertility via gut microbiota modulation." Chin J Nat Med. 2023 Jun;21(6):403-410. PMID: 37407171
- 9. Man Luo, Yuanyuan Chen, et al. "E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism." Front Immunol. 2023 May 19:14:1137089. PMID: 37275915
- 10. Li Zhang, Junjuan Lu, et al. "Akkermansia muciniphilaAmeliorates Lung Injury in Smoke-Induced COPD Mice by IL-17 and Autophagy." Cellular Microbiology. 15 March 2023.
- 11. Yao B, Cai Y, et al. "The Effect of Gut Microbiota on the Progression of Intervertebral Disc Degeneration." Orthop Surg 2023 Jan 04. PMID: 36600636
Related Biological Data

Related Biological Data

Solutions | Concentration | Storage |
HyperPFU™ high-fidelity DNA polymerase | 1,000 units/mL | -20°C |
5X HF Buffer | 5X | -20°C |
Product Source | An E. coli strain that carries the HyperPFU™ high-fidelity DNA polymerase gene. |
1. High fidelity—up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.
2. Enhanced robustness—fewer reaction failures and minimal optimization.
3. Improved yields—high product yields with minimal enzyme amounts (0.5–1 U/50 μL reaction).
4. High speed—increased processivity allows shorter reaction times (10X faster than Pfu).
5. Versatile – Can be used for routine PCR as well as long or difficult (GC-rich) templates.