Toggle Nav
Close
  • Menu
  • Setting

HyperPFU™ high-fidelity DNA polymerase

Catalog No.
K1031
High-fidelity Phusion DNA polymerase, used for PCR amplification of long fragments or difficult-to-amplify fragments (rich in GC)
Grouped product items
SizePriceStock Qty
100U
$55.00
In stock
500U
$140.00
In stock
1000U
$220.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

HyperPFU™ high-fidelity DNA polymerase is composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, it produces PCR products with very high speed and accuracy. Also, HyperPFU™ high-fidelity DNA polymerase is tolerant of various inhibitors, allowing for robust amplification with minimal optimization. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. It becomes the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes.
HyperPFU™ high-fidelity DNA polymerase is important for applications in which the DNA sequence needs to be correct after amplification. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. HyperPFU™ high-fidelity DNA polymerases is an ideal choice for cloning and can be used for long or difficult amplicons. It is one of the most accurate thermostable polymerases available, with an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase. It possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.
HyperPFU™ high-fidelity DNA polymerases is supplied with standard 5X HF Buffer, which can be used for complex or GC-rich templates.

Product Citation

Quality Control

Quality Control & DataSheet

View current batch:

Related Biological Data

Fusion high-fidelity DNA polymerase

Related Biological Data

Fusion high-fidelity DNA polymerase
 

Components and Storage

Solutions Concentration Storage
HyperPFU™ high-fidelity DNA polymerase 1,000 units/mL -20°C
5X HF Buffer 5X -20°C
Product Source An E. coli strain that carries the HyperPFU™ high-fidelity DNA polymerase gene.

Features

1. High fidelity—up to 50X more accurate than Taq; up to 6X more accurate than Pfu DNA polymerase.
2. Enhanced robustness—fewer reaction failures and minimal optimization.
3. Improved yields—high product yields with minimal enzyme amounts (0.5–1 U/50 μL reaction).
4. High speed—increased processivity allows shorter reaction times (10X faster than Pfu).
5. Versatile – Can be used for routine PCR as well as long or difficult (GC-rich) templates.

Storage

Store at -20°C.