In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Nitecapone is S-COMT inhibitor .
Catechol-O-methyltransferase (COMT) plays an essential role in normal brain function and has been implicated in human disorders, such as Parkinson's disease. The COMT has been involved in the degradation of catecholamines including dopamine, epinephrine, and norepinephrine. .
In vitro: Nitecapone is a highly effective inhibitor of rat S-COMT with IC50 values of about 300 nM in the liver and 20 nM in the brain tissues. The Ki value in the rat liver was 23 nM. In pure recombinant COMT enzyme forms, the Ki value of nitecapone was around 1 nM . Nitecapone was selective for COMT, inhibiting dopamine-β-hydroxylase, tyrosine hydroxylase, DOPA decarboxylase, and monoamine oxidase-A/B with IC50 values in the micromolar range .
In vivo: In rats, oral administration of nitecapone (3-30 mg/kg) in combination with levodopa and carbidopa effectively reduced 3-OMD formation and elevated serum and brain l-dopa, dopamine, DOPAC, and HVA levels. Nitecapone prevented ischemia-reperfusion injury in experimental heart surgery in rats. Nitecapone increased bicarbonate secretion from rat and human duodenum after both i.v. and intraluminal administration. High concentrations of nitecapone increased synthesis and secretion of gastric sulfomucin .
 Mnnist P T, Kaakkola S. Catechol-O-methyltransferase (COMT): biochemistry, molecular biology, pharmacology, and clinical efficacy of the new selective COMT inhibitors[J]. Pharmacological reviews, 1999, 51(4): 593-628.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; ≥3.64 mg/mL in EtOH with ultrasonic; ≥60.4 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|