Nile Red
Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.
Reference:
1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.
- 1. Qiushi Zheng, Chao Li, et al. "Candida auris cells form giant lipid droplets to survive in harsh environments." Commun Biol. 2025 May 22;8(1):783. PMID: 40404799
- 2. Badri Parshad, Andrew George Baker, et al. "Improved Therapeutic Efficiency of Senescent Cell-specific, Galactose-Functionalized Micelle Nanocarriers." SMALL. 18 December 2024
- 3. Athira Venugopal, Ronit Vogt Sionov, et al. "The V-Shaped Structuring Regulated via the LuxS-Dependent Quorum-Sensing Pathway Is Associated With Lactiplantibacillus plantarum Survivability in Acidic Environments." FOOD FRONTIERS. 25 November 2024
- 4. Nathanyel Sebbane, Itzhak Abramovitz, et al. "Mechanistic Insight into the Anti-Bacterial/Anti-Biofilm Effects of Low Chlorhexidine Concentrations on Enterococcus faecalis—In Vitro Study." Microorganisms 2024, 12(11), 2297
- 5. Jun Zhao, Rui Wang, et al. "Suppression of AQP7 is Crucial for Proliferation and Lipid Metabolism in ccRCC." Research Square. 13 Mar, 2024
- 6. Ronit Vogt Sionov, et al. "Anti-Bacterial and Anti-Biofilm Activities of Arachidonic Acid against the Cariogenic Bacterium Streptococcus mutans." Front Microbiol. 2024 Feb 26:15:1333274. PMID: 38596377
- 7. Ye Zhu, et al. "Metabarcoding Analysis of Microorganisms Inside Household Washing Machines in Shanghai, China." Microorganisms. 2024 Jan 13;12(1):160. PMID: 38257987
- 8. Goldie Wolfson, Ronit Vogt Sionov, et al. "Anti-Bacterial and Anti-Biofilm Activities of Anandamide against the Cariogenic Streptococcus mutans." Int J Mol Sci. 2023 Mar 24;24(7):6177. PMID: 37047147
| Storage | Store at -20°C |
| M.Wt | 318.37 |
| Cas No. | 7385-67-3 |
| Formula | C20H18N2O2 |
| Solubility | ≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one |
| SDF | Download SDF |
| Canonical SMILES | CCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
|
Cell lines |
Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein |
|
Reaction Conditions |
100 ng/ml Nile Red |
|
Applications |
Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm). |
|
Note |
The technical data provided above is for reference only. |
|
References: 1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973. |
|
Quality Control & MSDS
- View current batch:
Chemical structure
