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Nile Red

Catalog No.
B8209
A fluorescent stain specific for intracellular lipid droplets
Grouped product items
SizePriceStock Qty
10mg
$40.00
In stock
50mg
$65.00
In stock
100mg
$80.00
In stock
500mg
$140.00
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

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Background

Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red. 

Reference:

1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.

Chemical Properties

StorageStore at -20°C
M.Wt318.37
Cas No.7385-67-3
FormulaC20H18N2O2
Solubility≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
Chemical Name9-(diethylamino)-5H-benzo[a]phenoxazin-5-one
SDFDownload SDF
Canonical SMILESCCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein

Reaction Conditions

100 ng/ml Nile Red

Applications

Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm).

Note

The technical data provided above is for reference only.

References:

1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.

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