In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NECA is a non-selective agnonist of adenosine receptor with the concentration of 10 μM .
Adenosine is an important endogenous signaling molecule and plays a pivotal role in regulating a wide range of physiological functions, such as immune system response and inflammation, via cooperated with its receptor. It has been shown that adenosine receptor agonists can be either anti-inflammatory or proinflammatory, and the dual role of the adenosine receptor agonists causing these opposing effects should provide a better guide for therapeutic intervention used for different diseases in clinic .
When tested with primary murine microglia cells, NECA treatment significantly inhibited the productions of CXCL10 and TNF-αinduced by LPS [1, 3].
In CD73(-/-) mice model infected with T. gondii cysts, administration of NECA protected CD73(-/-) mice against T. gondii-induced immunopathology via agonist adenosine receptor, which suggested that CD73-generated adenosine was critical for immune regulation during T.gondii infection . In a mouse model with human uveitis, injection of NECA at an early stage after immunization with peptides 1-20 inhibited effect on both Th1 and Th17 responses [1, 4]. When tested with adult male Wistar rats of amygdala-kindled seizures, intravenous infusion of NECA after administration of PHT increased the PHT level in brain compared with control group by antognisting adenosine receptor thus strengthened the anticonvulsant properties of PHT against amygdala kindled seizures .
. Mahamed, D.A., L.E. Toussaint, and M.S. Bynoe, CD73-Generated Adenosine Is Critical for Immune Regulation during Toxoplasma gondii Infection. Infect Immun, 2015. 83(2): p. 721-9.
. Solomou, S. and M. Korbonits, The role of ghrelin in weight-regulation disorders: Implications in clinical practice. Hormones (Athens), 2014. 13(4): p. 458-475.
. Newell, E.A., et al., 2',3'-cAMP, 3'-AMP, 2'-AMP and adenosine inhibit TNF-alpha and CXCL10 production from activated primary murine microglia via A2A receptors. Brain Res, 2015. 1594: p. 27-35.
. Liang, D., et al., Anti-inflammatory or proinflammatory effect of an adenosine receptor agonist on the Th17 autoimmune response is inflammatory environment-dependent. J Immunol, 2014. 193(11): p. 5498-505.
. Sun, Z., et al., Activation of Adenosine Receptor Potentiates the Anticonvulsant Effect of Phenytoin Against Amygdala Kindled Seizures. CNS Neurol Disord Drug Targets, 2014.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in H2O; ≥15.35 mg/mL in DMSO; ≥2.61 mg/mL in EtOH with gentle warming and ultrasonic|
|Canonical SMILES||O[[email protected]]1[[email protected]@H](N2C3=NC=NC(N)=C3N=C2)O[[email protected]](C(NCC)=O)[[email protected]]1O|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while.|