In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: 0.20 μM: inhibits inducible nitric oxide synthases (iNOS).
IC50: 350 μM: blocks endothelial nitric oxide synthases (eNOS).
N-Benzylacetamidine is a potent and selective inhibitor of iNOS compared to eNOS. NOS is essential to the formation of nitric oxide (NO) via the NADPH- and O2-dependent oxidation of L-arginine, which plays a role in regulating many pathological and physiological processes, including smooth muscle relaxation and neurotransmission. INOS contributes to immune response and inflammation while eNOS plays a vital role in regulating vascular tone.
In vitro: N-Benzylacetamidine showed good iNOS and almost negligible eNOS inhibition in THP-1 cells, a human myelomonocytic leukemia cell line .
In vivo: Up to now, in vivo study of N-Benzylacetamidine is still in the development stage.
. Maccallini, C., Patruno, A., Besker, N., Ali, J., Ammazzalorso, A., & De Filippis, B. et al. Synthesis, Biological Evaluation, and Docking Studies of N-Substituted Acetamidines as Selective Inhibitors of Inducible Nitric Oxide Synthase. Journal of Medicinal Chemistry. 2009; 52(5): 1481-1485.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Formula||C9H12N2 · HBr|
|Solubility||≤25mg/ml in ethanol;15mg/ml in DMSO;20mg/ml in dimethyl formamide|
|Chemical Name||N-(phenylmethyl)-ethanimidamide, monohydrobromide|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while.|