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MTT

Catalog No.
B7777
reagent used in the measurement of in vitro cell proliferation
Grouped product items
SizePriceStock Qty
500mg
$32.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

IC50: N/A

MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) is a reagent used in the measurement of in vitro cell proliferation.

Tetrazolium salts have been the most widely used tools in cell biology for deternining the metabolic activity of cells ranging from microbial origin to mammalian. Fractionation studies in mammalian cells indicate that NADH, the reduced pyridine nucleotide cofactor, is responsible for most MTT reduction, which is further supported by studies with whole cells.

In vitro: It has been found that MTT reduction was associated not only with mitochondria, but also with the cytoplasm and nonmitochondrial membranes including endosome/lysosome compartment and plasma membrane. The net positive charge on MTT appears to be the predominant factor involved in their cellular uptake through the potential of plasma membrane. However, the second generation tetrazolium dyes (XTT, WST-1 and to some extent, MTS) that form water-soluble formazans and require an intermediate electron acceptor for reduction, are characterised by a net negative charge and therefore are largely cell-impermeable [1].

In vivo: MTT currently is only used for in vitro cell proliferation determination and there is no in vivo study reported.

Clinical trial: N/A

Reference:
[1] Berridge MV,Herst PM,Tan AS.  Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev.2005;11:127-52.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt414.32
Cas No.298-93-1
FormulaC18H16N5SBr
Solubility≥41.4mg/mL in DMSO
Chemical Name3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide
SDFDownload SDF
Canonical SMILESCC1=C(C)N=C([N+]2=NC(C3=CC=CC=C3)=NN2C4=CC=CC=C4)S1.[Br-]
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Chemical structure

MTT

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MTT