MMP-2 Inhibitor I
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
MMP-2 Inhibitor I, a hydroxamate-based, long-chain fatty acid, is a reversible inhibitor of matrix metalloproteinase (MMP)-2.
Matrix metalloproteinases (MMPs) have been involved in the degradation of extracellular matrix. MMPs contribute to long-term remodeling processes such as embryogenesis, tumor invasion, inflammation, angiogenesis, and wound healing. MMP-2, also known as gelatinase A or type IV collagenase, has been involved in regulating diverse cellular functions independent of its action on the extracellular matrix, including vascular tone, platelet aggregation, and mediation of the acute mechanical dysfunction of the heart immediately after ischemia and reperfusion . Up-regulation of MMP-2 has been associated with tumor invasion and metastasis .
MMP-2 Inhibitor I inhibited the activity of matrix metalloproteinase (MMP)-2 with the Ki value of 1.6 μM . MMP-2 Inhibitor I attenuated cancer cell migration . MMP-2 Inhibitor I had also been used to preserve blood-brain barrier function in a Wistar rat model of pneumococcal meningitis .
 Berton A, Rigot V, Huet E, et al. Involvement of fibronectin type II repeats in the efficient inhibition of gelatinases A and B by long-chain unsaturated fatty acids[J]. Journal of Biological Chemistry, 2001, 276(23): 20458-20465.
 Wang W, Schulze C J, Suarez-Pinzon W L, et al. Intracellular action of matrix metalloproteinase-2 accounts for acute myocardial ischemia and reperfusion injury[J]. Circulation, 2002, 106(12): 1543-1549.
 Emmert-Buck M R, Roth M J, Zhuang Z, et al. Increased gelatinase A (MMP-2) and cathepsin B activity in invasive tumor regions of human colon cancer samples[J]. The American journal of pathology, 1994, 145(6): 1285.
 Barichello T, Generoso J S, Michelon C M, et al. Inhibition of matrix metalloproteinases-2 and-9 prevents cognitive impairment induced by pneumococcal meningitis in Wistar rats[J]. Experimental Biology and Medicine, 2014, 239(2): 225-231.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Synonyms||Matrix Metalloproteinase-2 Inhibitor I,cis-9-Octadecenoyl-N-hydroxylamide,Oleylhydroxamate|
|Solubility||≤50mg/ml in ethanol;50mg/ml in DMSO;50mg/ml in dimethyl formamide|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|