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MHY1485mTOR activator, autophage inhibitor

MHY1485

Catalog No. B5853
Size Price Stock Qty
10mg $70.00 In stock
50mg $245.00 In stock
200mg $525.00 In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & MSDS

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Chemical structure

MHY1485

Protocol

Cell experiment [1]:

Cell lines

Ac2F cells

Preparation method

The solubility of this compound in DMSO is > 19.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

2 μM; 6 hrs

Applications

Under starvation, LC3 and lysosomal signal overlap in Ac2F cells transfected with AdGFP-LC3 and stained with LysoTracker (for visualization of lysosomes) was decreased by MHY1485. Thus, MHY1485 inhibited starvation-induced fusion between autophagosomes and lysosomes.

References:

[1]. Choi YJ, Park YJ, Park JY, Jeong HO, Kim DH, Ha YM, Kim JM, Song YM, Heo HS, Yu BP, Chun P, Moon HR, Chung HY. Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion. PLoS One. 2012;7(8):e43418.

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Chemical Properties

Cas No. 326914-06-1 SDF Download SDF
Chemical Name 4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
Canonical SMILES [O-][N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)=O
Formula C17H21N7O4 M.Wt 387.39
Solubility >19.4mg/mL in DMSO Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

Background

MHY1485 is a potent mTOR activator that inhibits autophagy with suppression of fusion between autophagosomes and lysosomes. [1]

mTOR (mechanistic target of rapamycin) is a serine/threonine kinase that regulates cellular metabolism, growth ad survival. It mediates the cellular response to stress, growth factor and hormone etc. Inhibition of mTOR strongly induced autophagy. mTOR signaling also plays a role in the promotion of ovarian follicle development.

In Ac2F rat hepatocytes, MHY1485 treatment suppresses the basal autophagic flux, and it also shows inhibitory effect under cell starvation which is an inducer of autophagy. The effects of MHY1485 dose-and time- dependently cause accumulation of LC3II and enlargement of the autophagosomes. [1]

Ovaries of juvenile mice cultured with MHY1485 for 4 days increases ovarian explant weights and follicle development. 2 days pre-incubation of these ovaries with MHY1485 followed by allo-grafting into kidney capsules of adult ovariectomized hosts for 5 days, shows apparent increases in graft weights and promotion of follicle development. [2]

References:
1, Choi YJ, Park YJ, Park JY et al.  Inhibitory effect of mTOR activator MHY1485 on autophagy: suppression of lysosomal fusion.  PLoS One. 2012;7(8):e43418. doi:10. 1371/journal.pone.0043418. Epub 2012 Aug 22. Erratum in: PLoS One. 2013;8(1).
2, Cheng Y, Kim J, Li XX, Hsueh AJ.  Promotion of ovarian follicle growth following mTOR activation: synergistic effects of AKT stimulators.  PLoS One. 2015 Feb 24;10(2):e0117769.