In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Methyllycaconitine citrate(MLA) is an antagonist of α7-containing neuronal nicotinic acetylcholine receptors (nAChRs; Ki = 1.4 nM)[1,2].
MLA inhibits the decreased cell viability induced by Aβ25-35, pretreatment with 5 and 10 µM. Aβ25-35 treatment increases LC3-II levels, which is inhibited by administration of Methyllycaconitine citrate. MLA also inhibits Aβ-induced autophagosome accumulation in SH-SY5Y cells.
MLA inhibits methamphetamine(METH)-induced climbing behavior by 50%. MLA prevents a decrease in striatal synaptosome dopamine (DA) uptake, MLA significantly attenuates METH-induced neurotoxicity at 72 h post-treatment. MLA fully prevents microglial activation at 24 h post-treatment and tending to confirm its neuroprotective activity.
. Kalappa B I, Sun F, Johnson S R, et al. A positive allosteric modulator of α7 nAChRs augments neuroprotective effects of endogenous nicotinic agonists in cerebral ischaemia. Brit.J.Pharmacol, 2013, 169(8): 1862-1878.
. Ward J M, Cockcroft V B, Lunt G G, et al. Methyllycaconitine: A selective probe for neuronal α-bungarotoxin binding sites. FEBS Lett, 1990, 270(1-2): 45-48 .
. Zheng X, et al. Methyllycaconitine alleviates amyloid-β peptides-induced cytotoxicity in SH-SY5Y cells. PLoS One, 2014, 9(10): e111536.
. Escubedo E, et al. Methyllycaconitine prevents methamphetamine-induced effects in mouse striatum: involvement of alpha7 nicotinic receptors. J Pharmacol Exp Ther, 2005, 315(2): 658-67.
|Physical Appearance||White solid|
|Storage||Desiccate at -20°C|
|Solubility||Soluble in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|