M-CSF, human recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Macrophage Colony stimulating Factor (M-CSF) is a hematopoietic growth factor produced by lymphocytes, monocytes, fibroblasts, macrophages, bone marrow stromal cells and endothelial cells. M-CSF plays important roles in regulating cell proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their respective progenitor cells. M-CSF also enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines from macrophages. MCSF plays critical roles in bone metabolism, immunological defenses, lipoproteins clearance, fertility and pregnancy.
The human M-CSF cDNA encodes a 554 aa protein contains a 32 aa signal sequence. Human M-CSF is active in the mouse, while mouse M-CSF is species-specific. Recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits.
|Determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells.|
|ED50||< 5.0 ng/ml.|
|Specific activity||> 2*105 units/mg.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:< 0.1 ng/μg
|Gene ID:||1435||Accession #:||P09603|
|Alternate Names:||Human M-CSF, M-CSF, MCSF, M CSF, h-M-CSF, rh-M-CSF, recombinant human M-CSF, recombinant M-CSF.|
|Source:||Escherichia Coli.||M.Wt:||36.8 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Sterile filtered and then lyophilized from 5 mM Tris, pH 8.0 + 80 mM Arginine.|
|Solubility:||Reconstitute in water to a concentration of 0.1-0.5 mg/ml. The solution can then be diluted into other aqueous buffers and stored at +4°C for 1 week and -20℃ for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|