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Levofloxacin

Catalog No.
B1959
synthetic fluoroquinolone antibiotic
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$50.00
In stock
5g
$90.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Background

Levofloxacin, a synthetic fluoroquinolone, is an antibacterial agent that inhibits the supercoiling activity of bacterial DNA gyrase, halting DNA replication.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt361.37
Cas No.100986-85-4
FormulaC18H20FN3O4
Solubility≥11.4mg/mL in DMSO
Chemical Name(S)-9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-3,7-dihydro-2H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid
SDFDownload SDF
Canonical SMILESFC1=C(C2=C(C3=C1[H])N(C([H])=C(C(O[H])=O)C3=O)[[email protected]](C([H])([H])[H])([H])C([H])([H])O2)N4C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])C4([H])[H]
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:

Cell lines

Osteoblastic cells

Preparation method

The solubility of this compound in DMSO is ≥11.4 mg/ml. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

80 μg/ml

Applications

Levofloxacin has the smallest inhibitory effect on osteoblast growth, with a concentration of approximately 80 mg / mL at 50% inhibition at 48 and 72 hours. Levofloxacin strongly inhibits calcium deposition, as can be determined by alizarin red staining and biochemical analysis on day 14.

Animal experiment [1]:

Animal models

Juvenile New Zealand White rabbits

Dosage form

100 mg/kg (p.o.), for 7 days

Application

Levofloxacin initially inhibited the synthesis of glycosaminoglycans, followed by the inhibition of DNA synthesis and mitochondrial function in cultured rabbit chondrocytes at actual arthritic concentrations, but these changes were reversible and were not sufficient to kill the cells.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Holtom PD, Pavkovic SA, Bravos PD, Patzakis MJ, Shepherd LE, Frenkel B.Inhibitory effects of the quinolone antibiotics trovafloxacin, ciprofloxacin, and levofloxacin on osteoblastic cells in vitro. J Orthop Res. 2000 Sep;18(5):721-7. PubMed PMID: 11117292.

[2]. Kato M, Takada S, Ogawara S, Takayama S. Effect of levofloxacin on glycosaminoglycan and DNA synthesis of cultured rabbit chondrocytes at concentrations inducing cartilage lesions in vivo. Antimicrob Agents Chemother.1995 Sep;39(9):1979-83. PubMed PMID: 8540702; PubMed Central PMCID: PMC162867.

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