L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase.
Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components.
In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities . In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice .
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
 Monis, B. ,Wasserkrug, H. and Seligman, A.M. Comparison of fixatives and substrates for aminopeptidase. Journal of Histochemistry and Cytochemistry 13(6), 503-509 (1965).
 Rüttger, A. ,Mollenhauer, J.,Lser, R., et al. Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. Biotechniques 41(4), 469-473 (2006).
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤25mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide|
|Chemical Name||(S)-2-amino-N-(4-methoxy-2-naphthalenyl)-4-methyl-pentanamide, monohydrochloride|
|Canonical SMILES||COC1=CC(NC([[email protected]](CC(C)C)N)=O)=CC2=CC=CC=C21.Cl|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|