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L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)

Catalog No.
C3708
substrate for aminopeptidase M and leucine aminopeptidase
Grouped product items
SizePriceStock Qty
100mg
$76.00
Ship with 5-10 days
250mg
$177.00
Ship with 5-10 days
1g
$599.00
Ship with 5-10 days

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

L-Leucine 4-methoxy-β-naphthylamide is a substrate for aminopeptidase M and leucine aminopeptidase.

Aminopeptidases are enzymes catalyzing the cleavage of amino acids from the amino terminus (N-terminus) of proteins or peptides. Aminopeptidases are distributed widely throughout the animal and plant kingdoms and are present in various subcellular organelles, in cytosol, as well as membrane components.

In vitro: L-Leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. In a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including L-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. Selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. This method was successfully validated by measurements in cells isolated from cathepsin B-/--, K-/--, and L-/-- mice [2].

In vivo: Up to now, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

References:
[1] Monis, B. ,Wasserkrug, H. and Seligman, A.M. Comparison of fixatives and substrates for aminopeptidase. Journal of Histochemistry and Cytochemistry 13(6), 503-509 (1965).
[2] Rüttger, A. ,Mollenhauer, J.,Lser, R., et al. Microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. Biotechniques 41(4), 469-473 (2006).

Chemical Properties

Physical AppearanceA crystalline solid
StorageStore at -20°C
M.Wt322.8
Cas No.4467-68-9
FormulaC17H22N2O2·HCl
SynonymsLeu-MNA
Solubility≤25mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide
Chemical Name(S)-2-amino-N-(4-methoxy-2-naphthalenyl)-4-methyl-pentanamide, monohydrochloride
SDFDownload SDF
Canonical SMILESCOC1=CC(NC([[email protected]](CC(C)C)N)=O)=CC2=CC=CC=C21.Cl
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.

Quality Control

Quality Control & MSDS

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Chemical structure

L-Leucine 4-methoxy-β-naphthylamide (hydrochloride)