L-a-Hydroxyglutaric acid disodium salt
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
L-α-Hydroxyglutaric acid disodium salt is a salt form of L-α-Hydroxyglutaric acid. L-α-Hydroxyglutaric acid accumulates as a result of a rare defect in L-2-HG dehydogenase, resulting in the metabolic disorder L-α-hydroxyglutaric aciduria.
In vitro: It has been reported that L-α-Hydroxyglutaric acid disodium salt could be used as the collection buffer for increasing the recovery of hypoxia-inducible factor-1 α, which was a marker of hypoxia in human tumors . In another previous study, L-α-Hydroxyglutaric acid disodium salt was also used in the radiolabeled conversion assay to evaluate its effect on the TET family of methyl hydroxylases .
In vivo: In the 30-day-old rats, L-α-Hydroxyglutaric acid was found to be able to significantly increase chemiluminescence, PCF and TBA-RS measurements and decrease the TAR values in cerebellum markedly. In addition, the L-α-Hydroxyglutaric acid-induced increase of TBA-RS was significantly attenuated by melatonin and also by the combinations of ascorbic acid and soluble alpha-tocopherol (Trolox) and of superoxide dismutase plus catalase but not by the inhibitor of nitric oxide synthase Nomega-nitro-L-arginine methyl ester (L-NAME), , superoxide dismutase or creatine or catalase alone in either cerebral structure .
Clinical trial: Up to now, L-a-Hydroxyglutaric acid disodium salt is still in the preclinical development stage.
 Park SR,Kinders RJ,Khin S,et al. Validation of a hypoxia-inducible factor-1 alpha specimen collection procedure and quantitative enzyme-linked immunosorbent assay in solid tumor tissues. Anal Biochem.2014 Aug 15;459:1-11.
 Evans B,Griner E. Registered report: Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Elife.2015 Jul 31;4:e07420.
 Latini A,Scussiato K,Rosa RB,Leipnitz G,Llesuy S,Belló-Klein A,Dutra-Filho CS,Wajner M. Induction of oxidative stress by L-2-hydroxyglutaric acid in rat brain. J Neurosci Res.2003 Oct 1;74(1):103-10.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥19.2mg/mL in H2O|
|Chemical Name||sodium (S)-2-hydroxypentanedioate|
|Canonical SMILES||O=C([O-])[[email protected]@H](O)CCC([O-])=O.[Na+].[Na+]|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|