In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Kartogenin is a potent inducer that promoting the differentiation of multipotent mesenchymal stem cells (MSCs) into chondrocytes with EC50 value of 100 nM .
MSCs are a kind of stem cell that can differentiate into different cell types including chondrocytes, osteoblasts, adipocytes and myocytes.
Kartogenin (KGN) potently induced the differentiation of hMSCs into chondrocytes in a dose-dependent way . In preskeletal mesenchymal cells from mice, KGN stimulated the formations of cartilage nodule and synovial joint, boosted digit cartilaginous anlaga elongation and stimulated interdigit invagination, tendon maturation and interzone compaction. KGN increased the expression of TGFβ1, which stimulated cartilage nodule formation. Also, KGN increased the phosphorylation of Smads that mediated BMP and TGFβ signaling .
In the acute surgical model and the chronic joint injury model, kartogenin showed chondroprotective effects. Kartogenin disrupted the interaction of filamin A and the transcription factor core-binding factor β subunit (CBFβ) and regulated the CBFβ-RUNX1 transcriptional, which then induced chondrogenesis . In the dermis of BALB/C mice, KGN induced synthesis of type-I collagen and activated Smad4/smad5 signaling pathway of the TGF-β .
. Johnson K, Zhu S, Tremblay MS, et al. A stem cell-based approach to cartilage repair. Science, 2012, 336(6082): 717-721.
. Decker RS, Koyama E, Enomoto-Iwamoto M, et al. Mouse limb skeletal growth and synovial joint development are coordinately enhanced by Kartogenin. Dev Biol, 2014, 395(2): 255-267.
. Wang J, Zhou J, Zhang N, et al. A heterocyclic molecule kartogenin induces collagen synthesis of human dermal fibroblasts by activating the smad4/smad5 pathway. Biochem Biophys Res Commun, 2014, 450(1): 568-574.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥10.6mg/mL in DMSO|
|Chemical Name||2-[(4-phenylphenyl)carbamoyl]benzoic acid|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|