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JE (MCP-1), murine recombinant protein

Catalog No.
P1034
JE (MCP-1), chemokine, expressed in E.Coli, active on human cells.
Grouped product items
SizePriceStock Qty
10ug
$238.00
Ship with 5-10 days
50ug
$653.00
Ship with 5-10 days
1mg
$3,630.00
Ship with 5-10 days

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Background

JE (MCP-1), also known as chemokine (C-C motif) ligand 2 (CCL2), is a member of the CC chemokine family that is a chemotactic agent for mononuclear cells. JE (MCP-1) is produced by endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as interleukin-1β (IL-1β), platelet derived growth factor (PDGF), CD40 ligand and oxidized low density lipoprotein. CCL2 induces histamine release by basophils, and enzyme and cytokine release by monocytes, NK cells and lymphocytes. CCL2-mediated recruitment of monocytes to sites of inflammation plays important roles in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.

 The murine JE (MCP-1) cDNA encodes a 148 aa protein with a 23 aa signal sequence. Human CCL2 induces a limited response in rodent cells, and mouse CCL2 has full activity on human cells. Recombinant murine JE (MCP-1) is a 13.8 kDa protein containing 125 amino acid residues.

Biological Activity

Determined by measuring the dose dependent chemotaxis with human THP-1 cells.
ED50 1-10 ng/ml.          

Quality Control

Quality Control & DataSheet

View current batch:
    Purity: ≥98%, by SDS-PAGE and HPLC.
    Endotoxin Level:<0.1 ng/μg

Related Biological Data

JE (MCP-1)
 

Description

Gene ID: 20296 Accession #: P10148
Alternate Names:Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11.
Source: Escherichia Coli. M.Wt: 13.8 kDa
Appearance: Lyophilized protein Storage: Store at -20°C
Formulation:Sterile filtered and lyophilized with no additives.
Solubility:Reconstitute in H2O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.
Shipping Condition: Blue ice. Handling: Centrifuge the vial prior to opening.
Usage:For Research Use Only! Not to be used in humans.