JE (MCP-1), murine recombinant protein
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
JE (MCP-1), also known as chemokine (C-C motif) ligand 2 (CCL2), is a member of the CC chemokine family that is a chemotactic agent for mononuclear cells. JE (MCP-1) is produced by endothelial cells, smooth muscle cells, and monocytes in response to several atherogenic stimulants such as interleukin-1β (IL-1β), platelet derived growth factor (PDGF), CD40 ligand and oxidized low density lipoprotein. CCL2 induces histamine release by basophils, and enzyme and cytokine release by monocytes, NK cells and lymphocytes. CCL2-mediated recruitment of monocytes to sites of inflammation plays important roles in the pathology of atherosclerosis, multiple sclerosis and allergic asthma.
The murine JE (MCP-1) cDNA encodes a 148 aa protein with a 23 aa signal sequence. Human CCL2 induces a limited response in rodent cells, and mouse CCL2 has full activity on human cells. Recombinant murine JE (MCP-1) is a 13.8 kDa protein containing 125 amino acid residues.
|Determined by measuring the dose dependent chemotaxis with human THP-1 cells.|
Quality Control & DataSheet
- View current batch:
Purity: ≥98%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
|Gene ID:||20296||Accession #:||P10148|
|Alternate Names:||Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11.|
|Source:||Escherichia Coli.||M.Wt:||13.8 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Sterile filtered and lyophilized with no additives.|
|Solubility:||Reconstitute in H2O to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use.|
|Shipping Condition:||Blue ice.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|