IFN-gamma, murine recombinant
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Interferon-gamma (IFN-γ) is a prototype proinflammatory cytokine that is mainly produced by activated T, B and NK cells. IFN-γ exists immunoregulatory activities, anti-viral activities, anti-proliferative and proinflammatory activities. IFN-γ can also upregulate MHC class I and II antigen expression by antigen-presenting cells. IFN-γ plays a key role in host defense by promoting the development and activation of Th1 cells, up-regulation of antigen presentation molecules, chemoattraction and activation of monocytes and macrophages and immunoglobulin class switching in B cells. In addition, IFN-γ functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation.
Mature mouse IFN-γ exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits. Recombinant murine IFN-γ is a 15.6 kDa protein containing 134 amino acid residues.
|Determined by a cytopathic affect inhibition assay with murine NIH 3T3 cells challenged with VSV virus.|
|ED50||< 0.1 ng/ml.|
|Specific activity||> 1*107 units/mg.|
Quality Control & DataSheet
- View current batch:
Purity: ≥95%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
|Gene ID:||15978||Accession #:||P01580|
|Alternate Names:||IFNG, Murine IFN-γ, IFN-γ, IFNγ, IFN γ, m- IFN-γ, rm- IFN-γ, recombinant murine IFN-γ, recombinant IFN-γ, IFN.|
|Source:||Escherichia Coli.||M.Wt:||15.6 kDa|
|Appearance:||Lyophilized protein||Storage:||Store at -20°C|
|Formulation:||Lyophilized from 10 mM Na2PO4, pH 7.0 + 25 mM NaCl.|
|Solubility:||Reconstitute in ddH2O to a concentration of 1 mg/ml. Further dilutions should be made into RPMI + 10% FCS and stored at 4℃ for 1 week or -20℃ for future use.|
|Shipping Condition:||Gel pack.||Handling:||Centrifuge the vial prior to opening.|
|Usage:||For Research Use Only! Not to be used in humans.|