HyperChrom Butyl HP Agarose
HyperChrom Butyl HP Agarose is a hydrophobic interaction chromatography medium that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This series of chromatography media can be well used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
HyperChrom Butyl HP Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, resulting in high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide. (4) Fine particle size design to improve resolution.
HyperChrom Butyl HP Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Hydrophobic interactions |
|
Ligation |
R-O-CH2-CH(OH)-CH2-O-(CH2)3-CH3 |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
34 μm |
|
Ligand density |
~50 μmol Butyl group/mL chromatography medium |
|
Dynamic load |
~38 mg β-lactoglobulin/mL chromatography medium |
|
Flow rates are recommended |
90-150 cm/h |
|
Maximum flow rate |
200 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability* |
3-13 |
|
Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 1 M NaOH, 1 M acetic acid, 6 M guanidine hydrochloride, 8 M urea |
|
Intolerant to solvents |
Oxidant |
|
* After the chromatography medium was placed at 40 °C and pH 3-13 for 7 days, its physicochemical properties and functions did not change significantly. **30% and 75% are v/v, volume ratio. |
|
| Components | PC2021-25 mL | PC2021-100 mL |
| HyperChrom Butyl HP Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||