HyperChrom Butyl 4FF Agarose
HyperChrom Butyl 4FF Agarose is a hydrophobic interaction chromatography medium that takes advantage of differences in the hydrophobic properties and the amount of different molecules under specific conditions. This series of chromatography media can be well used for the isolation and purification of a variety of biomolecules, such as recombinant proteins, antibodies, viral vaccines, etc.
HyperChrom Butyl 4FF Agarose has excellent scale-up performance: (1) Highly crosslinked agarose base frame with excellent rigidity, so high process flow rates and improved process efficiency at low backpressure. (2) Hydrophilic base frame to minimize the influence on the hydrophobic role of the ligand. (3) Through chemical modification, it has excellent chemical compatibility and is resistant to CIP cleaning such as sodium hydroxide.
HyperChrom Butyl 4FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Hydrophobic interactions |
|
Ligation |
R-O-CH2-CH(OH)-CH2-O-(CH2)3-CH3 |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
40 μmol Butyl group/mL chromatography medium |
|
Flow rates are recommended |
150-250 cm/h |
|
Maximum flow rate |
400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4-30℃ |
|
pH stability* |
2-14 |
|
Solvent resistant |
Common aqueous solution, 3 M ammonium sulfate, 30% isopropanol**, 75% ethanol**, 1 M NaOH, 6 M guanidine hydrochloride |
|
Intolerant to solvents |
oxidant |
|
* After 7 days of storage of chromatography medium at 40oC and pH 2-14 environment, its physicochemical properties and functions did not change significantly. **30% and 75% are v/v, volume ratio. |
|
| Components | PC2020-25 mL | PC2020-100 mL |
| HyperChrom Butyl 4FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||