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Catalog No.
C-Raf inhibitor,potent and selective
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
In stock
In stock
In stock
Ship with 10-15 days

Tel: +1-832-696-8203

Email: [email protected]

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GW5074 is a synthetic inhibitor of c-Raf with IC50 value of 9nM [1].

GW5074 is found to be a potent inhibitor of c-Raf and has no effect on cdk1, cdk2, c-src, p38 MAP kinase, VEGFR2 and c-fms. GW5074 also shows no direct effect on JNK proteins and p38 MAP kinase. In neurons, GW5074 reduces the phosphorylation level of c-Raf at Ser259, results in an induction of c-Raf activity. It is further proved that GW5074 shows neuroprotective efficacy when its dose is below 1μM. In addition, GW5074 suppresses cell apoptosis through delaying the down-regulation of Akt phosphorylation and preventing the activation of GSK3β. In the in vivo model of Huntington’s disease, GW5074 protects neurons via resisting 3-NP-induced striatal neurodegeneration [1].

Our product is a mixture of Z and E configuration.

[1] Burgess S, Echeverria V. Raf inhibitors as therapeutic agents against neurodegenerative diseases. CNS & Neurological Disorders-Drug Targets (Formerly Current Drug Targets-CNS & Neurological Disorders), 2010, 9(1): 120-127.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
Cas No.220904-83-6
Solubilityinsoluble in EtOH; insoluble in H2O; ≥21.9 mg/mL in DMSO
Chemical Name3-(3,5-dibromo-4-hydroxybenzylidene)-5-iodoindolin-2-one
SDFDownload SDF
Canonical SMILESIC1=CC=C(NC(C2=CC3=CC(Br)=C(O)C(Br)=C3)=O)C2=C1
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while.


Cell experiment [1]:

Cell lines

Cerebellar granule neurons

Preparation method

The solubility of this compound in DMSO is >26.1mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

1 μM, 40 min


Treatment of neurons with GW5074 caused c-Raf activation and stimulated the Raf-MEK-ERK pathway. Treatment of neurons with GW5074 led increased the activity of B-Raf. GW5074 inhibited cell death caused by neurotoxins in granule cells and other neuronal types.

Animal experiment [1,2]:

Animal models

C57BL/6 male mice with Huntington’s disease (HD), Mice repeatedly exposed to smoke from four cigarettes each day for four weeks

Dosage form

Intraperitoneal injection, 2 mg/kg, every day for three weeks


GW5074 (5 mg/kg) completely prevented extensive bilateral striatal lesions induced by 3-NP in C57BL/6 male mice. GW5074 (0.5 mg/kg or 2 mg/kg) suppressed side stream smoke-induced airway hyperresponsiveness in mice.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.


[1]. Chin P C, Liu L, Morrison B E, et al. The c‐Raf inhibitor GW5074 provides neuroprotection in vitro and in an animal model of neurodegeneration through a MEK‐ERK and Akt‐independent mechanism[J]. Journal of neurochemistry, 2004, 90(3): 595-608.

[2]. Lei Y, Cao Y X, Xu C B, et al. The Raf-1 inhibitor GW5074 and dexamethasone suppress sidestream smoke-induced airway hyperresponsiveness in mice[J]. Respiratory research, 2008, 9(1): 71.

Biological Activity

Description GW5074 is a potent and selective inhibitor of c-Raf with an IC50 value of 9 nM.
Targets C-Raf          
IC50 9 nM          

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