Ethacrynic Acid
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Ethacrynic acid is one of the most used loop diuretics, acting by inhibiting NKCC2 which is specifically found in the thick ascending loop of Henle in the kidneys. Inhibition of NKCC2 significantly increases the concentrations of Na+, K+, and Cl− in the tubule, and reduces hypertonicity in the surrounding interstitium, causing less water to be reabsorbed into the blood. Eventually, more urine will be produced and excreted, and a decrease in blood volume can be achieved. Loop diuretics have been used for the treatment of fluid overload/edema in congestive heart failure, acute pulmonary edema, nephrotic syndrome, hepatic ascites, renal failure, as well as other edematous disorders.
References:
1. Vardanyan R, Hruby V. Synthesis of Best-Seller Drugs. Chapter 21 - Diuretics, 2016, 317-327.
2. Zins GR, Walk RA, Gussin RZ, et al. The diuretic activity of ethacrynic acid in rats. Journal of Pharmacology and Experimental Therapeutics, 1968, 163(1): 210-215.
| Storage | Store at -20°C |
| M.Wt | 303.14 |
| Cas No. | 58-54-8 |
| Formula | C13H12Cl2O4 |
| Synonyms | MK 595,NSC 85791,NSC 624008 |
| Solubility | insoluble in H2O; ≥15.15 mg/mL in DMSO; ≥44.4 mg/mL in EtOH |
| Chemical Name | 2-(2,3-dichloro-4-(2-methylenebutanoyl)phenoxy)acetic acid |
| SDF | Download SDF |
| Canonical SMILES | O=C(O)COC1=CC=C(C(C(CC)=C)=O)C(Cl)=C1Cl |
| Shipping Condition | Ship with blue ice, or upon other requests. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
| Animal experiment:[2] | |
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Animal models |
Rats |
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Dosage form |
37.5 and 125 mg/kg Intravenous administration |
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Applications |
Single doses of 37.5 and 125 mg/kg increased water and potassium excretion 2-fold, sodium excretion 3-fold and chloride excretion 4-fold in relation to parallel control animals. Single dose activity was confined to the 1st hr after injection whereas effects persisted for the duration of observation when animals were primed with 25 mg/kg and sustained with 50 mg/kg/hr. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Vardanyan R, Hruby V. Synthesis of Best-Seller Drugs. Chapter 21 - Diuretics, 2016, 317-327. 2. Zins GR, Walk RA, Gussin RZ, et al. The diuretic activity of ethacrynic acid in rats. Journal of Pharmacology and Experimental Therapeutics, 1968, 163(1): 210-215. |
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Quality Control & MSDS
- View current batch:
-
Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
