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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EGTA, also known as egtazic acid, is an aminopolycarboxylic acid, a calcium chelator. It can protects against cell death caused by nitric oxide-induced calcium influx into nerve cells[1,2].
References:
[1]. Boullerne A I, Nedelkoska L, Benjamins J A. Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes. Journal of Neuroscience Research, 2001, 63(2): 124-135.
[2]. Fisher A E O, Hague T A, Clarke C L, et al. Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG. Biochemical and Biophysical Research Communications, 2004, 323(1): 0-167.
