In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
IC50: Not available.
EDC.HCl, which is commonly regarded as carbodiimide reagents, serves as a very useful tool for the formation of amide bonds (peptide bonds) in an aqueous medium. As one of the carbodiimide reagents, it is also effectively applied in anhydroxydation, polynucleotide synthesis, esterification and lactonization.In addition, EDC.HCl is also widely used as water soluble condensing reagent. 
In vitro: Carbodiimide-mediated coupling method was one of the most traditional approaches for peptide-bond formation. With the aim to synthesis target compounds, carbodiimide reagents were added after thoroughly mixing of a carboxylic acid and an amine. After the reaction was accomplished, the carbodiimide reagent was then changed into its corresponding urea.  Recently, a simple and fast detection method using spectrophotometric flow injection analysis was developed. This method was based on the reaction between EDC.HCl and pyridine in acidic aqueous solution. After reaction at 40C for a while, the absorbance was measured at 400 nm. The calibration curve demonstrated a good linearity from 0 to 10% of EDC·HCl solutions with the following regression equation: y = 3.15 × 104x (x, % concentration of EDC·HCl; y, peak area). This approach served to monitoring the concentration of EDC·HCl after its reaction in water. 
In vivo: So far, no in vivo data has been reported.
Clinical trial: So far, no clinical trial has been conducted.
Kunihiko Seno, Kazuki Matumura, Mitsuko Oshima, and Shoji Motomizu. Spectrophotometric determination of 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr; 24: 505-8.
 Jad YE, Khattab SN, Beatriz G. Torre DL, Govender T, Kruger HG, Faham AE and Albericio F. EDC·HCl and potassium salts of oxyma and oxyma-B as superior coupling cocktails for peptide synthesis. Eur J Org Chem. 2015 May; 2015(14): 3116-20.
|Physical Appearance||A solid|
|Storage||Desiccate at -20°C|
|Solubility||≥19.2 mg/mL in DMSO, ≥39.6 mg/mL in ETOH, ≥39 mg/mL in H2O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
Quality Control & MSDS
- View current batch:
Purity = 98.00%
- COA (Certificate Of Analysis)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)