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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
E. coli uracil-DNA glycosylase (UDG) is expressed by recombinant E. coli strains cloned with the E. coli UDG gene and purified in multiple steps. E. coli UDG catalyzes the hydrolysis of N-glycosidic bonds between uracil bases and deoxyribose in single- or double-stranded DNA, releasing free uracil, but not from 6-base or shorter oligonucleotides. Inactive to RNA.
Components
1000 U
5000 U
Uracil-DNA Glycosylase (UDG) (5 U/µL)
200 μL
1 mL
10 X UDG Reaction Buffer
1.5 mL
Store the components at -20°C for 2 years.