DMPO

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
DMPO is a kind of water soluble nitric oxide spin trap, which allows the measurement of oxygen-centered free radicals in biological systems at room temperature using electron spin resonance (ESR). [1]
It has a high reaction rate constant for superoxide and hydroxyl radicals, and distinguishes simultaneously among a variety of important biologically generated free radicals. [1][2]
In vitro, It was demonstrated in red blood cells and in hamster V79 cells that the DMPO spin adducts of ·O2− and ·OH are metabolized very rapidly so that even if formed, they may not be detected in many experiments with cells.[1]
In vivo, when DMPO perfused the heart at 100 μm/L, it attenuated the development of reperfusion arrhythmias during the first 10 min of reperfusion and improved the functional recovery of the heart during reperfusion. Without treatment, 55% of hearts showed irreversible ventricular fibrillation, and this was completely prevented by DMPO, due to its function of traping·O2− and ·OH radicals.[2]
References:
[1] Samuni A1, Samuni A, Swartz HM. The cellular-induced decay of DMPO spin adducts of ·O2− and ·OH. Free Radic Biol Med. 1989; 6(2):179-83.
[2] Tosaki A1, Blasig IE, Pali T, et al. Heart protection and radical trapping by DMPO during reperfusion in isolated working rat hearts. Free Radic Biol Med. 1990; 8 (4):363-72.
Physical Appearance | A colorless oil |
Storage | Store at -20°C |
M.Wt | 113.16 |
Cas No. | 3317-61-1 |
Formula | C6H11NO |
Solubility | ≥11.3 mg/mL in DMSO; ≥55.9 mg/mL in H2O; ≥65.8 mg/mL in EtOH |
Chemical Name | 2,2-dimethyl-3,4-dihydro-2H-pyrrole 1-oxide |
SDF | Download SDF |
Canonical SMILES | [O-][N+]1=CCCC1(C)C |
Shipping Condition | Ship with blue ice, or upon other requests. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Chemical structure

Related Biological Data
