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Cy7.5 azide (non-sulfonated)

Catalog No.
used for Click chemistry to label alkyne-modified oligonucleotides
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Ship with 5-10 days
Ship with 5-10 days

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Cy7.5 azide a NIR fluorescence dye that ready for the use in Click Chemistry labeling with long-wave infrared fluorescence. For other fluorescent applications, especially requiring low fluorescent background, this labeling reagent was also popular used. As generic Click Chemistry labeling protocol, azide is available as DMSO solution. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

Cy7.5 azide a sulfonated dye with absorption peaks at 773 and 808 nm, can be the optimal fluorescent probe that should have separate absorption spectra. The changes in the absorption and scattering properties of the tissue are negligible in the range of 750 to 850 nm [1].

[1] Osnat Harbater,Israel Gannot.  Fluorescent probes concentration estimation in vitro and ex vivo as a model for early detection of Alzheimer’s disease.Jornal of biomedical optics. 2014.19(12).

Chemical Properties

Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Solubilitysoluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Chemical NameN-(3-azidopropyl)-6-[(2E)-1,1-dimethyl-2-[(2E)-2-[3-[(E)-2-(1,1,3-trimethylbenzo[e]indol-3-ium-2-yl)ethenyl]cyclohex-2-en-1-ylidene]ethylidene]benzo[e]indol-3-yl]hexanamide
SDFDownload SDF
Canonical SMILESCC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)C)C=CC4=CC(=CC=C5C(C6=C(N5CCCCCC(=O)NCCCN=[N+]=[N-])C=CC7=CC=CC=C76)(C)C)CCC4)C
Shipping ConditionShip with blue ice, or upon other requests.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while.

Spectral properties

Excitation max (nm) 788 Emission max (nm) 808
Extinction Coefficient (M-1cm-1) 223000 Quantum Yield N/A
CF260 N/A CF280 N/A

Quality Control

Quality Control & MSDS

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Chemical structure

Cy7.5 azide (non-sulfonated)

Related Biological Data

Cy7.5 azide (non-sulfonated)