|Cy5 amine (non-sulfonated)
can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.
Sample solution is provided at 25 µL, 10mM.
|Cas No.||SDF||Download SDF|
|Solubility||>48mg/ml in DMSO||Storage||Store at -20°C|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Excitation max (nm)||646||Emission max (nm)||662|
|Extinction Coefficient (M-1cm-1)||250000||Quantum Yield||0.2|
Non-sulfonated cyanines are available for the labeling of many targets including:
|• Soluble proteins, which are tolerant to addition of organic co-solvent||• Fluorescence polarization (FP)|
|• Antibodies (with 5-10% of DMSO/DMF)||• Fluorescence resonance energy transfer (FRET)|
|• DNA and oligonucleotides||• Time-resolved fluorescence energy transfer (TR-FRET)|
|• Peptides||• Fluorescence intensity (FI)|
|• Many small molecules|
|• Reactions in organic media (dichloromethane, acetonitrile)|
Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths .
Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.