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Cy5 amine (non-sulfonated)
can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.

Catalog No.A8143
Size Price Stock Qty
Cy5 amine 5mg
$210.00
In stock
Cy5 amine 25mg
$410.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Qin Jiang, Zimiao Luo, et al. "Red blood cell membrane-camouflaged melanin nanoparticles for enhanced photothermal therapy." Biomaterials. S0142-9612(17)30483-0.

Quality Control

Chemical structure

Sanguinarine chloride

Related Biological Data

Sanguinarine chloride

Related Biological Data

Sanguinarine chloride

Cy5 amine (non-sulfonated) Dilution Calculator

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Chemical Properties

Cas No. SDF Download SDF
Chemical Name 1-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium
Canonical SMILES CC1(C)C2=C(C=CC=C2)[N+](CCCCCC(NCCCCCC[NH3+])=O)=C1/C=C/C=C/C=C3C(C)(C)C4=CC=CC=C4N\3C
Formula C38H54Cl2N4O M.Wt 653.77
Solubility >48mg/ml in DMSO Storage Store at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 646 Emission max (nm) 662
Extinction Coefficient (M-1cm-1) 250000 Quantum Yield 0.2
CF260 0.03 CF280 0.04

Application

Non-sulfonated cyanines are available for the labeling of many targets including:

• Soluble proteins, which are tolerant to addition of organic co-solvent • Fluorescence polarization (FP)
• Antibodies (with 5-10% of DMSO/DMF) • Fluorescence resonance energy transfer (FRET)
• DNA and oligonucleotides • Time-resolved fluorescence energy transfer (TR-FRET)
• Peptides • Fluorescence intensity (FI)
• Many small molecules  
• Reactions in organic media (dichloromethane, acetonitrile)  

Background

Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].

Reference:
[1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.