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Cy5 amine (non-sulfonated)

Catalog No.
A8143
can be conjugated with reactive groups such as NHS esters, carboxy groups (after carbodiimide activation), and epoxides.
Grouped product items
SizePriceStock Qty
5mg
$210.00
In stock
25mg
$410.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Cy5 amine is a reactive dye which contains amino group. This dye can be coupled with a variety of activated esters and other electrophilic reagents. For instance, this amine can be coupled with EDC-activated carboxylic groups. With this bright and photostable reagent, many different methods of fluorescence detection is available and colorful fluorophore can also be easily detected in small quantities by naked human eye. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In A2780 cells, the conjugates polymer 2P-Cy3-Cy5 can be used for the analysis of the pair-correlation functions between Cy3 and Cy5. Treated with 2P-Cy3-Cy5 for several hours show similar shape and characteristic decay lengths [1].

Reference:
[1]Hartley, J. M.; Zhang, R.; Gudheti, M.; Yang, J.; Kopeek, J. Tracking and quantifying polymer therapeutic distribution on a cellular level using 3D dSTORM. Journal of Controlled Release, 2016, 231, 50–59.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt653.77
FormulaC38H54Cl2N4O
Solubility≥48 mg/mL in DMSO; insoluble in H2O; ≥8.84 mg/mL in EtOH
Chemical Name1-(6-((6-ammoniohexyl)amino)-6-oxohexyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3H-indol-1-ium
SDFDownload SDF
Canonical SMILESCC1(C)C2=C(C=CC=C2)[N+](CCCCCC(NCCCCCC[NH3+])=O)=C1/C=C/C=C/C=C3C(C)(C)C4=CC=CC=C4N\3C
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Spectral properties

Excitation max (nm) 646 Emission max (nm) 662
Extinction Coefficient (M-1cm-1) 250000 Quantum Yield 0.2
CF260 0.03 CF280 0.04

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