Cy3 carboxylic acid (non-sulfonated)
Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.
In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1].
Reference:
[1] Xue, M. ; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085–3093.
| Physical Appearance | A solid |
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 493.08 |
| Formula | C30H37ClN2O2 |
| Solubility | ≥49.3 mg/mL in DMSO; insoluble in H2O; ≥99 mg/mL in EtOH |
| Chemical Name | 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride |
| SDF | Download SDF |
| Canonical SMILES | CC(C1=C(N/2C)C=CC=C1)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC=C4)=C4C3(C)C.[Cl-] |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 555 | Emission max (nm) | 570 |
| Extinction Coefficient (M-1cm-1) | 150000 | Quantum Yield | 0.31 |
| CF260 | 0.04 | CF280 | 0.09 |
Quality Control & MSDS
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Chemical structure
Related Biological Data
