Cy5 NHS ester
Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:
[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
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| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 753.88 |
| Cas No. | 146368-14-1 |
| Formula | C37H43N3O10S2 |
| Synonyms | Sulfo-Cyanine 5 NHS ester; Sulfo-Cy5 NHS ester; CY5-SE; Sulfo-Cyanine5 Succinimidyl Ester |
| Solubility | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
| Chemical Name | 2-((1E,3E)-5-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| Canonical SMILES | CC[N+]1=C(/C=C/C=C/C=C2N(CCCCCC(ON3C(CCC3=O)=O)=O)C(C=CC(S(O)(=O)=O)=C4)=C4C/2(C)C)C(C)(C)C5=C1C=CC(S([O-])(=O)=O)=C5 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 646 | Emission max (nm) | 662 |
| Extinction Coefficient (M-1cm-1) | 271000 | Quantum Yield | 0.28 |
| CF260 | 0.04 | CF280 | 0.04 |
| Filter Set | Cy5 | Laser | 633-647 nm |
Quality Control & MSDS
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