Cy3 NHS ester
Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Sulfo-Cy3 NHS ester is ideal for proteins with low solubility and proteins prone to denaturation. Non-sulfonated Cy3 NHS ester is also available. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In vitro, sulfo-Cy3 NHS ester can react with green-emitting QDs ligand-exchanged and LA/His-PIMA-PEG-NH2 to provide QD-dye conjugates [1].
Reference:
[1] Reid, D. A.; Keegan, S.; Leo-Macias, A.; Watanabe, G.; Strande, N.T.; Chang, H.H.; Oksuz, B.A.; Fenyo, D.; Lieber, M.R.; Ramsden, D.A.; Rothenberg, E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(20).
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| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 727.84 |
| Cas No. | 146368-16-3 |
| Formula | C35H41N3O10S2 |
| Solubility | ≥51.5mg/ml in EtOH; ≥4.37 mg/ml in DMSO; ≥10.24mg/ml in H2O |
| Chemical Name | 2-((E)-3-((E)-1-(6-((2,5-dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| Canonical SMILES | O=C(ON1C(CCC1=O)=O)CCCCCN(C(C=CC(S(=O)(O)=O)=C2)=C2C/3(C)C)C3=C\C=C\C4=[N+](CC)C(C=CC(S(=O)([O-])=O)=C5)=C5C4(C)CC)C)C)C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 548 | Emission max (nm) | 563 |
| Extinction Coefficient (M-1cm-1) | 162000 | Quantum Yield | 0.1 |
| CF260 | 0.03 | CF280 | 0.06 |
Quality Control & MSDS
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Chemical structure
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