Cy3 azide
Sulfo-Cy3 azide is a sulfonated, hydrophilic and highly water soluble dye for Click Chemistry. For labeling of various molecules in aqueous phase for brightness and photostability of fluorophore, sulfo-Cy3 azide is an ideal choice. Click reaction can be carried out in pure water without need to use organic co-solvent. For labeling proteins and even intact biological objects, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In vitro, sulfo-Cy3 azide was used for fluorescent microscopy staining. Human U87MG glioblastoma cells that overexpress uPAR were just stained by Cy3-AE105 conjugate [1].
Reference:
[1] Sun, L. Gai, Y. Anderson, C.J.; Zeng, D. Highly-efficient and versatile fluorous-tagged Cu (I)-catalyzed azide–alkyne cycloaddition ligand for preparing bioconjugates. Chemical Communications, 2015, 51, 17072–17075.
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| M.Wt | 712.88 |
| Formula | C34H44N6O7S2 |
| Solubility | ≥10 mg/mL in DMSO; ≥16.67 mg/mL in EtOH; ≥16.67 mg/mL in H2O |
| Chemical Name | 1-(6-((3-azidopropyl)amino)-6-oxohexyl)-2-((E)-3-((E)-1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | O=C(NCCCN=[N+]=[N-])CCCCC[N+]1=C(/C=C/C=C2C(C)(C)C3=CC(S(=O)(O)=O)=CC=C3N\2CC)C(C)(C)C4=CC(S(=O)([O-])=O)=CC=C41 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 563 | Emission max (nm) | 584 |
| Extinction Coefficient (M-1cm-1) | 162000 | Quantum Yield | 0.1 |
| CF260 | 0.03 | CF280 | 0.06 |
Quality Control & MSDS
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Chemical structure
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