HyperFluor™ 488 azide
Catalog No.
A8118
Equivalent to Alexa Fluor 488 azide, reacts with alkynes via click chemistry.
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HyperFluor™ 488 azide is a green fluorescent dye conjugate used in Click chemistry. It can interaction with alkyne-based derivatives of biomolecules in the Click chemical reaction, results in the formation of stable compounds in the presence of a copper (I) catalyst (with terminal alkynes) or no catalyst (containing cyclooctyne). Due to this property, dyes can be used to label a variety of biomolecules, including proteins and antibodies. HyperFluor™ 488 molecular conjugates have high brightness and photostability, and are commonly used in flow cytometry and fluorescence microscopy for highly sensitive detection of biomolecules.
Excitation light (λex): 494 nm
Emitted light (λem): 517 nm
| Physical Appearance | lyophilized (freeze-dried) powder |
| Storage | Store at -20°C, protect from light |
| M.Wt | 658.66 |
| Cas No. | 1006592-63-7 |
| Formula | C27H26N6O10S2 |
| Synonyms | HyperFluor? 488 azide |
| Solubility | Soluble in DMSO |
| Chemical Name | 6-amino-9-(4-((6-azidohexyl)carbamoyl)-2-carboxyphenyl)-3-iminio-5-sulfo-3H-xanthene-4-sulfonate--6-amino-9-(5-((6-azidohexyl)carbamoyl)-2-carboxyphenyl)-3-iminio-5-sulfo-3H-xanthene-4-sulfonate (1/1) |
| Canonical SMILES | [NH2+]=C1C=CC2=C(C3=C(C(O)=O)C=C(C(NCCCCCCN=[N+]=[N-])=O)C=C3)C4=CC=C(N)C(S(O)(=O)=O)=C4OC2=C1S(=O)([O-])=O.[NH2+]=C5C=CC6=C(C7=C(C(O)=O)C=CC(C(NCCCCCCN=[N+]=[N-])=O)=C7)C8=CC=C(N)C(S(O)(=O)=O)=C8OC6=C5S(=O)([O-])=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 494 | Emission max (nm) | 517 |
| Extinction Coefficient (M-1cm-1) | 71800 | Quantum Yield | 0.91 |
| CF260 | 0.16 | CF280 | 0.1 |
| Filter Set | FITC / GFP | Laser | 488 nm |
Quality Control & MSDS
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Chemical structure
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