N3-kethoxal

N3-kethoxal is a small, membrane-permeable probe, which selectively binds to un-paired guanine residues within a cellular environment and then provides a bio-orthogonal handle for click chemistry reactions. Depending on how the azide group is utilized, N3-kethoxal is amenable to various nucleic acid probing applications. The N3-kethoxal platform can be utilized to probe RNA secondary and tertiary structure, map single stranded DNA regions in the genome, explore RNA-RNA interactions, and identify protein-RNA interactions. Compared with current nucleic acid probes, N3-kethoxal exhibits low toxicity, as well as high specificity, resolution, and reproducibility, providing a novel click chemistry platform for in vitro and in vivo analyses of nucleic acid structure and proximity interactions. 

References:

1. Huang J, Zhao R, Mo J, et al. N3-kethoxal-based bioorthogonal intracellular RNA labeling. ChemBioChem, 2021, 22(9): 1559-1562.

2. He C. Novel click chemistry platform for in vivo and in vitro analysis of nucleic acid structure and proximity interactions. Polsky Center for Entrepreneurship and Innovation, 2019, reference ID: 19-T-037.

3. Weng X, Gong J, Chen Y, et al. Keth-seq for transcriptome-wide RNA structure mapping. Nature Chemical Biology, 2020, 16(5): 489-492.