Biotin alkyne

Biotin alkyne is a click chemistry reagent that combines biotin and an alkyne functional group, primarily used in the field of biomarking and detection. It efficiently and specifically reacts with molecules containing azide groups via click chemistry, particularly the copper-catalyzed azide-alkyne cycloaddition (CuAAC), forming stable triazole bonds. This reaction proceeds under mild conditions, making it highly suitable for labeling biomolecules in physiological environments. In terms of applications, leveraging the extremely high-affinity binding between biotin and streptavidin or avidin, Biotin alkyne is widely employed for tracking, detecting, and enriching biomolecules such as proteins and nucleic acids. Its key features include high reaction efficiency and specificity, as well as the strong signal amplification provided by the biotin-avidin system. As a result, it serves as an important tool reagent in various research areas such as bioassays, protein purification, and targeted drug delivery. This reagent is typically supplied as a solid powder and should be stored at -20°C, protected from light and moisture.