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Cy3 hydrazide (non-sulfonated)

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carbonyl-reactive dye
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Cy3 hydrazide is a carbonyl-reactive dye, an analog of Cy3® hydrazide.

Cyanine3 dyes have been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc.

Cy3 hydrazide can be used to label various carbonyl-containing molecules such as antibodies and other glycoproteins after periodate oxidation, proteins which have undergone deamination or oxidative stress, or reducing saccarides. Cyanine3 is compatible with a number of fluorescent instruments.

Pan D, Qin H, Cooperman BS.  Synthesis and functional activity of tRNAs labeled with fluorescent hydrazides in the D-loop. RNA. 2009 Feb;15(2):346-54.
Tamarit J, de Hoogh A, Obis E, et al.  Analysis of oxidative stress-induced protein carbonylation using fluorescent hydrazides. J Proteomics. 2012 Jun 27;75(12):3778-88.
Kameyama A, Kaneda Y, Yamanaka H, et al.  Detection of oligosaccharides labeled with cyanine dyes using matrix-assisted laser desorption/ionization mass spectrometry. Anal Chem. 2004 Aug 1;76(15):4537-42.

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
Solubility≥54.4mg/mL in DMSO, ≥48.2 mg/mL in EtOH, ≥50.8 mg/mL in H2O
Chemical Name1-(6-hydrazinyl-6-oxohexyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride hydrochloride
SDFDownload SDF
Canonical SMILESCN1C2=CC=CC=C2C(C)(C)/C1=C\C=C\C3=[N+](CCCCCC(NN)=O)C4=CC=CC=C4C3(C)C.[Cl-].Cl
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

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Chemical structure

Cy3 hydrazide