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Cy3 carboxylic acid (non-sulfonated)
Non-reactive fluorophore, for control experiments, and for calibration

Catalog No.A8132
Size Price Stock Qty
5mg
$210.00
In stock
25mg
$410.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Chemical structure

Cy3 carboxylic acid (non-sulfonated)

Related Biological Data

Cy3 carboxylic acid (non-sulfonated)

Cy3 carboxylic acid (non-sulfonated) Dilution Calculator

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Chemical Properties

Cas No. N/A SDF Download SDF
Synonyms N/A
Chemical Name 1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride
Canonical SMILES CC(C1=C(N/2C)C=CC=C1)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]
Formula C30H37ClN2O2 M.Wt 493.08
Solubility ≥49.3mg/mL in DMSO Storage 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 555 Emission max (nm) 570
Extinction Coefficient (M-1cm-1) 150000 Quantum Yield 0.31
CF260 0.04 CF280 0.09

Application

Non-sulfonated cyanines are available for the labeling of many targets including:

• Soluble proteins, which are tolerant to addition of organic co-solvent • Fluorescence polarization (FP)
• Antibodies (with 5-10% of DMSO/DMF) • Fluorescence resonance energy transfer (FRET)
• DNA and oligonucleotides • Time-resolved fluorescence energy transfer (TR-FRET)
• Peptides • Fluorescence intensity (FI)
• Many small molecules  
• Reactions in organic media (dichloromethane, acetonitrile)  

Background

Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1].

Reference:
[1] Xue, M. ; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085–3093.