In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Target: superoxide anion
IC50: 69 μM (rat hepatocytes cells)
The small molecule coelenterazine, a luminescent enzyme substrate, can react with the reactive oxygen species (ROS) superoxide anion and peroxynitrite and produce a chemiluminescent signal . Therefore, coelenterazine is used for monitoring reporter genes in ELISA, BRET, and HTS techniques. Reactive oxygen species (ROS) play an important role in tumor development and survival and it is a new treatment strategy to target the cancer-specific ROS.
In vitro: Coelenterazine showed antioxidative properties in primary cultures of rat hepatocytes subjected to the oxidant tert-butyl hydroperoxide (t-BHP). Coelenterazine (3-10 μM) concentration-dependently increased survival and decreased lipid peroxidation in rat hepatocytes treated for 6 hr with t-BHP (250 μM) . In addition, Coelenterazine (10 μmol/l) can be used to assay superoxide anion concentrations in cancer cell lines .
In vivo: the small molecule coelenterazine (5 mg/kg, intravenous administration) was a potential reporter of cancer-associated superoxide anion in the 4T1 breast adenocarcinoma mouse model . Gli36-Rluc or Gli36-Gluc cells were injected into the leg muscle of nude mice. The mice were injected with 4 mg/kg of coelenterazine and imaged by a cooled charge-coupled device camera .
1. Bronsart LL, Stokes C, Contag CH. Multimodality Imaging of Cancer Superoxide Anion Using the Small Molecule Coelenterazine. Mol Imaging Biol. 2016;18(2):166-71.
2. Dubuisson ML, de Wergifosse B, Trouet A, Baguet F, Marchand-Brynaert J, Rees JF. Antioxidative properties of natural coelenterazine and synthetic methyl coelenterazine in rat hepatocytes subjected to tert-butyl hydroperoxide-induced oxidative stress. Biochem Pharmacol. 2000;60(4):471-8.
3. Morse D, Tannous BA. A water-soluble coelenterazine for sensitive in vivo imaging of coelenterate luciferases. Mol Ther. 2012;20(4):692-3.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≥42.3mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|