Chlorambucil

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Chlorambucil is a nitrogen mustard alkylating agent [1]
Chlorambucil is a chemotherapy medication commonly used for the treatment of chronic lymphocytic leukemia (CLL). Chlorambucil forms intra- and inter-strand crosslinks in DNA, the latter of which prevents DNA replication and transcription leading to cell death [1]
In embryonic mouse limb buds directly exposed to chlorambucil at 1 ~ 2 μg/mL, the number of affected cells reached a plateau at 48 hr after treatment and decreased significantly by 72 hr, at which time exposure to a second dose of chlorambucil showed little or no effect. Chlorambucil-induced cell death was restricted almost exclusively to regions of undifferentiated mesenchymal cells [2].
In CLL patients, chlorambucil treatment (0.9 mg/kg given over 3 days) induced greater decrease in lymphocyte count against the control group [3]. In pharmacokinetic studies in tumor-bearing dogs receiving 4 mg/m2 metronomic chlorambucil, the LD50 and IC50 of chlorambucil were 270 μM and 114 μM for SF767 cells, and 390 μM and 96 μM for U87-MG cells, respectively. In human microvascular endothelial cells and human progenitor derived endothelial colony forming cells, the IC50 of chlorambucil was 0.53 μM and 145 μM, correspondingly [4].
References:
[1]. Mattes W B, Hartley J A, Kohn K W. DNA sequence selectivity of guanine-N7 alkylation by nitrogen mustards. Nucleic Acids Research, 1986, 14(7): 2971-2987.
[2]. Sadler T W, Kochhar D M. Chlorambucil-induced cell death in embryonic mouse limb buds. Toxicology and Applied Pharmacology, 1976, 37(2): 237-256.
[3]. Begleiter A, Lee K, Israels L G, et al. Chlorambucil induced apoptosis in chronic lymphocytic leukemia (CLL) and its relationship to clinical efficacy. Leukemia, 1994, 8(1): S103-S106.
[4]. Reese M J, Knapp D W, Anderson K M, et al. In vitro effect of chlorambucil on human glioma cell lines (SF767 and U87-MG), and human microvascular endothelial cell (HMVEC) and endothelial progenitor cells (ECFCs), in the context of plasma chlorambucil concentrations in tumor-bearing dogs. PLoS One, 2018, 13(9): e0203517.
Physical Appearance | A solid |
Storage | Store at -20°C |
M.Wt | 304.21 |
Cas No. | 305-03-3 |
Formula | C14H19Cl2NO2 |
Solubility | insoluble in H2O; ≥12.15 mg/mL in DMSO; ≥17.7 mg/mL in EtOH |
SDF | Download SDF |
Canonical SMILES | ClCCN(CCCl)C(C=C1)=CC=C1CCCC(O)=O |
Shipping Condition | Ship with blue ice, or upon other requests. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. |
Quality Control & MSDS
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Chemical structure
