Canine EGF / Epidermal Growth Facto Protein (His Tag)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
|Protein Construction||A DNA sequence encoding the mature form of canine EGF (Q9BEA0) (Asn 973-Arg 1024) was fused with a polyhistidine tag at the N-terminus.|
|Purity||> 98 % as determined by SDS-PAGE|
|Bio-activity||Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts.
The EC50 for this effect is typically 0.1-0.5 ng/ml.
|Endotoxin||Please contact us for more information.|
|Stability||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal||Met|
|Molecular Mass||The recombinant canine EGF consists of 68 amino acids and has a calculated molecular mass of 8.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of canine EGF is approximately 12 kDa.|
|Formulation||Lyophilized from sterile PBS, pH7.5
|Storage||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
|MSTN / GDF8 Protein Description||
Canine epidermal growth factor (EGF), also known as urogastrone (URG) since it was isolated from urine based on its inhibitory effect on gastric secretion, is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. EGF is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble EGF is a 6 kDa peptide corresponding to the EGF unit located proximal to the transmembrane domain. EGF is expressed in kidney, salivary gland, cerebrum and prostate, and plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. As a potent mitogen, EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro. Furthermore, EGF is also identified as a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4), a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion.