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Canine EGF / Epidermal Growth Facto Protein (His Tag)

Catalog No.G1006
Size Price Stock Qty
500µg
$180.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Description

Synonym CEGF
Protein Construction A DNA sequence encoding the mature form of canine EGF (Q9BEA0) (Asn 973-Arg 1024) was fused with a polyhistidine tag at the N-terminus.
Source Canine
Expression Host E.coli
Purity > 98 % as determined by SDS-PAGE
Bio-activity Measured in a cell proliferation assay using Balb 3T3 mouse embryonic fibroblasts.
The EC50 for this effect is typically 0.1-0.5 ng/ml.
Endotoxin Please contact us for more information.
Stability Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal Met
Molecular Mass The recombinant canine EGF consists of 68 amino acids and has a calculated molecular mass of 8.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of canine EGF is approximately 12 kDa.
Formulation Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Storage Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
MSTN / GDF8 Protein Description
Canine epidermal growth factor (EGF), also known as urogastrone (URG) since it was isolated from urine based on its inhibitory effect on gastric secretion, is the founding member of the EGF-family of growth factors. All the members of this protein family have highly similar structural and functional characteristics. EGF is initially synthesized as a 130 kDa single-pass transmembrane protein containing 9 EGF-like units and 9 LDL-receptor class B repeats. The mature soluble EGF is a 6 kDa peptide corresponding to the EGF unit located proximal to the transmembrane domain. EGF is expressed in kidney, salivary gland, cerebrum and prostate, and plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. As a potent mitogen, EGF stimulates the growth of various epidermal and epithelial tissues both in vivo and in vitro. Furthermore, EGF is also identified as a magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4), a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion.