In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
AS1842856 is a specific Foxo1 inhibitor with IC50 of 30 nM. AS1842856 effectively inhibits autophagy. AS1842856 only reduces Foxo1 activity by binding to Foxo1 without affecting its transcription and protein expression.
AS1842856 (0.1 μM) inhibits 70% of Foxo1-mediated promoter activity. AS1842856 may inhibit the activity of endogenous G6Pase and PEPCK by reducing the mRNA levels of G6Pase and PEPCK, which may inhibit the production of glucose in Fao cells.
In db/db mutant mice with diabetes, oral administration of AS1842856 can inhibit the genes related to gluconeogenesis of the liver, resulting in a large decrease in fasting blood glucose. In normal mice and db/db mice, treatment with AS1842856 can also suppress the increase in blood glucose levels caused by injection of pyruvate.
. Nagashima T, et al. Discovery of novel forkhead box O1 inhibitors for treating type 2 diabetes: improvement of fasting glycemia in diabetic db/db mice. Mol Pharmacol, 2010, 78(5): 961-70.
. He J, et al. The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway. Biosci Rep, 2019, 39(5).
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in EtOH; insoluble in H2O; ≥11.75 mg/mL in DMSO with gentle warming|
|Chemical Name||5-amino-7-(cyclohexylamino)-1-ethyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|