ARCA Cy3 EGFP mRNA (5-moUTP)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ARCA Cy3 EGFP mRNA (5-moUTP) can be used to analyze mRNA delivery and translation efficiency. The EGFP mRNA will express an enhanced version of green fluorescent protein, which was originally isolated from the jellyfish, Aequorea victoria. EGFP is a direct detection reporter gene commonly used in mammalian cell cultures to produce bright green fluorescence with an emission peak at 509 nm. EGFP mRNA labeled with cyanine 5 can be directly visualized. ARCA Cy3 EGFP mRNA (5-moUTP) is an ideal molecule for determining mRNA delivery and localization and is independent of translation.
Cyanine 3 is a synthetic fluorescent dye with maximum excitation and emission wavelengths of 550 nm and 570 nm, respectively. APExBIO ARCA Cy3 EGFP mRNA (5-moUTP) is transcribed in a ratio of 1:3 with Cyanine 3-UTP: 5-methoxy-UTP. Substitution in this ratio results in mRNA that is easy to visualize and can still be translated in cell culture. Translation efficiency is the opposite of Cyanine 3-UTP replacement.
ARCA Cy3 EGFP mRNA (5-moUTP) is restricted using APExBIO's proprietary co-transcriptional capping method to produce a naturally occurring Cap 0 structure with high end capping efficiency. It is polyadenylation, modified with 5-methoxyuridine and optimized for mammalian systems. It mimics the fully processed mature mRNA.
|mRNA Length||996 nucleotides|
|Buffer||1 mM Sodium Citrate, pH 6.4||Storage||-40°C or below|
|General tips||Dissolve it on ice and take care to prevent RNase contamination degradation. Avoid repeated freezing and thawing as much as possible. Do not vortex. For the first time, it is gently centrifuged and divided into several parts for stand-alone use. Use RNase-free reagents and consumables, using appropriate RNase-free technology. It can not be added to the serum-containing medium until it is mixed with the transfection reagent.|
|Shipping Condition||Trial Pack: Blue Ice Transport.|