In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aphidicolin, a tetracyclic diterpenoid, is an antibiotic with novel structure produced by the mold Cephalosporium aphidicola. Aphidicolin is an inhibitor of DNA polymerase-α .
DNA polymerase-α is an enzyme complex involved in initiation of DNA replication and during synthesis of Okazaki fragments on the lagging strand. The DNA polymerase-α complex consists of 4 subunits: POLA1, POLA2, PRIM1, and PRIM2. POLA1 is the catalytic subunit. POLA2 is the regulatory subunit. PRIM1 and PRIM2 are the small and the large primase subunits .
Aphidicolin selectively inhibited the activity of DNA polymerase-α without activity for DNA polymerase-β and mitochondrial DNA polymerase. Aphidicolin prevented cell division in sea urchin embryos . Aphidicolin was a potent inhibitor of cellular deoxyribonucleic acid synthesis. Aphidicolin strongly inhibited the growth of herpes simplex virus both in tissue culture and in the rabbit eye. Aphidicolin was active against iododeoxyuridine-resistant herpes virus . Aphidicolin showed antiviral activity and inhibited the incorporation of thymidine into DNA of cultured human embryonic lung cells. Aphidicolin (15 μg/ml) reduced the activity of crude and partially purified DNA polymerases from the cytosol .
 Ikegami S, TAGUCHI T, OHASHI M, et al. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-α[J]. Nature, 1978, 275(5679): 458-460.
 Lehman I R, Kaguni L S, Krause K H, et al. DNA polymerase alpha[J]. J. Biol. Chem, 1989, 264.
 Bucknall R A, Moores H, Simms R, et al. Antiviral effects of aphidicolin, a new antibiotic produced by Cephalosporium aphidicola[J]. Antimicrobial agents and chemotherapy, 1973, 4(3): 294-298.
 Ohashi M, Taguchi T, Ikegami S. Aphidicolin: a specific inhibitor of DNA polymerases in the cytosol of rat liver[J]. Biochemical and biophysical research communications, 1978, 82(4): 1084-1090.
|Physical Appearance||A white to off-|
|Storage||Store at -20°C|
|Solubility||≥33.3mg/mL in DMSO|
|Canonical SMILES||O[[email protected]@]1(CC[[email protected]@]23[[email protected]@]([H])(CC[[email protected]]4([[email protected]@](C)([[email protected]@H](CC[[email protected]@]43C)O)CO)[H])C[[email protected]@H]1C2)CO|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|