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ApexPrep DNA Plasmid Miniprep Kit

Catalog No.
A5001
used for isolation of plasmid DNA
Grouped product items
SizePriceStock Qty
50 tests
$40.00
In stock
300 tests
$200.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

The ApexPrep DNA Plasmid Miniprep kit is based on alkaline lysis technology, which can lyse cells and then adsorb DNA to the adsorption membrane in the presence of high salt. Subsequent steps such as washing away impurities can efficiently and quickly extract up to 30 μg of plasmid DNA. This kit uses a unique buffer formula to maximize the removal of protein impurities and other organic compounds, and can process 1-5 mL of bacterial solution each time. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics, and other factors.

Plasmid DNA prepared by ApexPrep DNA Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and trans-formation, in vitro translation, and transfection of robust cells.

Product Citation

Chemical Properties

StorageStore at room temperature for one year
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & DataSheet

View current batch:
 

Components and Storage

Components 50 tests 300 tests
Buffer BL 30 mL 160 mL
Buffer A1 20 mL 100 mL
Buffer A2 20 mL 100 mL
Buffer A3 30 mL 120 mL
Buffer AP 12 mL 50 mL
Buffer AE 15 mL 30 mL
RNase A 200 μL (10mg/mL) 1 mL (10mg/mL)
Spin Columns 50 300
Collection Tubes 50 300

Store at room temperature for one year. Buffer A1 with RNase A should be stored at 2-8°C.

Description

For purification of up to 20 μg molecular biology grade plasmid DNA

  • Ready-to-use plasmid DNA in minutes
  • Reproducible yields of molecular biology grade plasmid DNA
  • Single protocol for high- and low-copy vectors

The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.