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ApexPrep DNA Plasmid Miniprep Kit
used for isolation of plasmid DNA

Catalog No.A5001
Size Price Stock Qty
(50)
$40.00
In stock
(300)
$200.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

      

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Wang EX, Ding MZ, et al. "Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21. PMID:26809519

Description

For purification of up to 20 μg molecular biology grade plasmid DNA

  • Ready-to-use plasmid DNA in minutes
  • Reproducible yields of molecular biology grade plasmid DNA
  • Single protocol for high- and low-copy vectors

The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.

 

DNA Miniprep Protocol Using a Microcentrifuge
1.    Inoculate 1-5 ml LB medium (selective antibiotic added) with a single colony, incubate with vigorous shaking at 37 °C overnight.
2.    Harvest bacteria by centrifuging at > 6000 rpm for 5 min. Remove supernatant and keep pellet.
3.    Resuspend bacterial pellet with 250 μl Buffer P1 and transfer to a fresh microcentrifuge tube.
4.    Add 250 μl Buffer P2 and mix gently by inverting 5-6 times at room temperature.
5.    Add 350 μl Buffer P3 and mix gently by inverting 5-6 times. Spin down at 12000 rpm for 10 min to remove bacterial/protein debris. Transfer supernatant to a spin column.
6.    Centrifugation at 6000 rpm for 1 min. Discard flow-through.
7.    (Optional), wash spin column with 0.5 ml Buffer PB; centrifuge at 6000 rpm for 1 min, and discard flow-through.
8.    Wash spin column with 0.75 ml Buffer PE; centrifuge at 6000 rpm for 1 min, and discard flow-through.
9.    Centrifuge at 12000 rpm for an additional 1 min to remove residual wash buffer.
10.    To elute DNA, place the column in a clean 1.5 ml collection tube. Add 50 μl Buffer EB to the center of the membrane, let stand for 1 min, and centrifuge at 12000 rpm for 1 min to collect eluted DNA.