ApexPrep DNA Plasmid Miniprep Kit
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
The ApexPrep DNA Plasmid Miniprep kit is based on alkaline lysis technology, which can lyse cells and then adsorb DNA to the adsorption membrane in the presence of high salt. Subsequent steps such as washing away impurities can efficiently and quickly extract up to 30 μg of plasmid DNA. This kit uses a unique buffer formula to maximize the removal of protein impurities and other organic compounds, and can process 1-5 mL of bacterial solution each time. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics, and other factors.
Plasmid DNA prepared by ApexPrep DNA Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and trans-formation, in vitro translation, and transfection of robust cells.
- 1. Xiang Song Jian Gong, et al. "Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay." Br J Cancer. 2020 Oct;123(9):1437-1444. PMID:32782293
- 2. Wang EX, Ding MZ, et al. "Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21. PMID:26809519
| Storage | Store at room temperature for one year |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
| Components | 50 tests | 300 tests |
| Buffer BL | 30 mL | 160 mL |
| Buffer A1 | 20 mL | 100 mL |
| Buffer A2 | 20 mL | 100 mL |
| Buffer A3 | 30 mL | 120 mL |
| Buffer AP | 12 mL | 50 mL |
| Buffer AE | 15 mL | 30 mL |
| RNase A | 200 μL (10mg/mL) | 1 mL (10mg/mL) |
| Spin Columns | 50 | 300 |
| Collection Tubes | 50 | 300 |
Store at room temperature for one year. Buffer A1 with RNase A should be stored at 2-8°C. | ||
For purification of up to 20 μg molecular biology grade plasmid DNA
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
