ApexPrep DNA Plasmid Miniprep Kit
The ApexPrep DNA Plasmid Miniprep kit is based on alkaline lysis technology, which can lyse cells and then adsorb DNA to the adsorption membrane in the presence of high salt. Subsequent steps such as washing away impurities can efficiently and quickly extract up to 30 μg of plasmid DNA. This kit uses a unique buffer formula to maximize the removal of protein impurities and other organic compounds, and can process 1-5 mL of bacterial solution each time. The yield and quality of plasmid extraction are related to the type of host bacteria and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics, and other factors.
Plasmid DNA prepared by ApexPrep DNA Plasmid Miniprep Kit is suitable for a variety of routine applications including restriction enzyme digestion, sequencing, library screening, ligation and trans-formation, in vitro translation, and transfection of robust cells.
- 1. Xiang Song Jian Gong, et al. "Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay." Br J Cancer. 2020 Oct;123(9):1437-1444. PMID:32782293
- 2. Wang EX, Ding MZ, et al. "Reorganization of a synthetic microbial consortium for one-step vitamin C fermentation." Microb Cell Fact. 2016 Jan 25;15:21. PMID:26809519
| Components | 50 tests | 300 tests |
| Buffer BL | 30 mL | 160 mL |
| Buffer A1 | 20 mL | 100 mL |
| Buffer A2 | 20 mL | 100 mL |
| Buffer A3 | 30 mL | 120 mL |
| Buffer AP | 12 mL | 50 mL |
| Buffer AE | 15 mL | 30 mL |
| RNase A | 200 μL (10mg/mL) | 1 mL (10mg/mL) |
| Spin Columns | 50 | 300 |
| Collection Tubes | 50 | 300 |
Store at room temperature for one year. Buffer A1 with RNase A should be stored at 2-8°C. | ||
For purification of up to 20 μg molecular biology grade plasmid DNA
- Ready-to-use plasmid DNA in minutes
- Reproducible yields of molecular biology grade plasmid DNA
- Single protocol for high- and low-copy vectors
The ApexPrep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications such as fluorescent and radioactive sequencing and cloning.
