In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Anhydrotetracycline (hydrochloride) (ATC) is a powerful effector for the tetracycline repressor (TetR) and reverse tetracycline repressor (revTetR) systems .
Tetracycline repressor (TetR) is an effector-regulated DNA-binding protein that binds tightly to its palindromic tetO operator DNA in the absence of effector, thereby blocking the transcription of any downstream genes. Binding of tetracycline (TC) or anhydrote-tracycline (ATC) to TetR causes the repressor to dissociate from the DNA and gene transcription can occur. Contrary to TetR, reverse tetracycline repressor (revTetR) mutant binds tetO only in the presence of ATC .
Anhydrotetracycline (hydrochloride) is a powerful effector for TetR and revTetR systems. Anhydrotetracycline (ATC) bound TetR with a 500-fold higher affinity and represented the most efficient inducer . ATC bound the Tet repressor 35-fold more potent than Tet . However, ATC acted as a corepressor in revTetR variant. In β-galactosidase (β-gal) assays, TetR inhibitedβ-gal expression to nearly 1%, while ~100% expression was accomplished in the presence of 0.4 μM ATC. RevTetR produced almost 100%β-gal activity in the absence of ATC, while the presence of 0.4 μM ATC resulted in a 5-fold decrease ofβ-gal activity .
. Gossen M, Bujard H. Anhydrotetracycline, a novel effector for tetracycline controlled gene expression systems in eukaryotic cells. Nucleic Acids Res. 1993 Sep 11;21(18):4411-2.
. Kamionka A, Bogdanska-Urbaniak J, Scholz O, et al. Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor. Nucleic Acids Res. 2004 Feb 4;32(2):842-7.
. Resch M, Striegl H, Henssler EM, et al. A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR. Nucleic Acids Res. 2008 Aug;36(13):4390-401.
. Degenkolb J, Takahashi M, Ellestad GA, et al. Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor. Antimicrob Agents Chemother. 1991 Aug;35(8):1591-5.
|Storage||Store at -20°C|
|Solubility||≥15.4mg/mL in EtOH with gentle warming; ≥12.5 mg/ml in H2O|
|Canonical SMILES||CN(C)[[email protected]@H]1C(=C(C(=O)N)C(=O)[[email protected]]2(O)[[email protected]]1Cc1c(C)c3cccc(O)c3c(O)c1C2=O)OCl|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|